Isolation of basal cells and submucosal gland duct cells from mouse trachea.
Isolation of basal cells and submucosal gland duct cells from mouse trachea.
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DOI：
10.3791/3731
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发表时间：
2012-09-14
期刊：
影响因子：
--
通讯作者：
Gomperts BN
中科院分区：
文献类型：
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作者：
Hegab AE;Ha VL;Attiga YS;Nickerson DW;Gomperts BN
大气道直接与环境接触，因此容易受到我们吸入的毒素和感染剂的伤害。因此，大气道需要有效的修复机制来保护我们的身体。这种修复过程发生在气道中的干细胞中，从气道中分离这些干细胞对于理解修复和再生的机制很重要。这对于理解可能导致气道疾病的异常修复也很重要。该方法的目的是从小鼠气管粘膜下腺导管中分离出一种新的干细胞群，并将这些细胞置于体外和体内模型系统中，以鉴定粘膜下腺的修复和再生机制。这一成果展示了可用于分离和测定大气道中的导管和基底干细胞的方法，这将使我们能够研究气道疾病，如囊性纤维化、哮喘和慢性阻塞性肺病。目前，还没有分离粘膜下腺管细胞的方法，也没有研究粘膜下腺再生的体内模型。
The large airways are directly in contact with the environment and therefore susceptible to injury from toxins and infectious agents that we breath in . The large airways therefore require an efficient repair mechanism to protect our bodies. This repair process occurs from stem cells in the airways and isolating these stem cells from the airways is important for understanding the mechanisms of repair and regeneration. It is also important for understanding abnormal repair that can lead to airway diseases . The goal of this method is to isolate a novel stem cell population from the mouse tracheal submucosal gland ducts and to place these cells in in vitro and in vivo model systems to identify the mechanisms of repair and regeneration of the submucosal glands . This production shows methods that can be used to isolate and assay the duct and basal stem cells from the large airways .This will allow us to study diseases of the airway, such as cystic fibrosis, asthma and chronic obstructive pulmonary disease. Currently, there are no methods for isolation of submucosal gland duct cells and there are no in vivo models to study the regeneration of submucosal glands.