Expression of Nrf2 in Different Cells after Human Cerebral Cortex Contusion.
Expression of Nrf2 in Different Cells after Human Cerebral Cortex Contusion.
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DOI：
10.12116/j.issn.1004-5619.2019.03.002
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发表时间：
2019-06-01
期刊：
影响因子：
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通讯作者：
Zhao, R
中科院分区：
文献类型：
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作者：
Guo, X S;Wen, S H;Zhao, R
摘要：目的观察人大脑皮质挫伤后不同时间点不同细胞核因子-红细胞衍生因子2相关因子（Nrf 2）的表达变化，探讨其在脑损伤年龄推断中的应用。方法选取3 - 6例脑组织，其中6例为对照组，30例为脑挫裂伤后不同时间点的脑组织，分为伤后0-1 h、3-6 h、1-3 d、5-7 d、10-14 d组，每组6例。石蜡包埋切片，HE染色观察损伤后形态学变化，免疫荧光双标法检测神经元、星形胶质细胞和小胶质细胞中Nrf 2的表达。计数阳性细胞数并进行统计学分析。结果伤后1- 3d，神经元数目减少。神经元损伤后Nrf 2表达增加，阳性细胞率在损伤后1- 3d达高峰。损伤后1- 3d胶质细胞被激活，5-7 d达到高峰。伤后10-14 d开始出现软化点。胶质细胞酸性蛋白（GFAP）阳性细胞在伤后逐渐增多，在伤后5-7 d达到高峰，而Nrf 2在GFAP阳性细胞中所占比例相对稳定。损伤后，离子化钙结合适配分子1（IBA 1）阳性细胞逐渐增多并活化。Nrf 2在IBA 1阳性细胞中的表达比例逐渐增加，在伤后5-7 d达高峰，随后下降。结论Nrf 2在不同细胞中的表达参与了损伤后不同细胞的生物学功能，单个细胞的动态表达具有时间依赖性。这为脑挫伤伤龄的推断提供了一个新的参考指标。
ABSTRACT: Objective To observe the expression changes of nuclear factor-erythroid derived 2-related factors （Nrf2） in different cells at different time points after human cerebral cortex contusion, and to discuss its application in brain wound age estimation. Methods Thirty-six human brain tissues were selected, of which 6 were for control and 30 were cortical contusion at different time points post-injury, which were divided into 0-1 h, 3-6 h, 1-3 d, 5-7 d, and 10-14 d post-injury groups, with 6 cases in each group. Based on paraffin embedded sections, HE staining was used to observe the morphological changes post-injury, and double immunofluorescence staining was used to detect the expression of Nrf2 in neurons, astrocytes, and microglia. The number of positive cells was counted and statistical analysis was made. Results The number of neurons decreased 1-3 d post-injury. The expression of Nrf2 cells in neurons increased after injury, and the rate of positive cells peaked at 1-3 d post-injury. Glial cells were activated 1-3 d post-injury, and the activation peaked at 5-7 d post-injury. The cerebromalacia began to form at 10-14 d post-injury. Glial fibrillary acidic protein （GFAP） positive cells in mice increased gradually after injury and peaked at 5-7 d post-injury, while the proportion of Nrf2 in GFAP positive cells was relatively stable. After injury, ionized calcium-binding adapter molecule 1 （IBA1） positive cells increased and activated gradually. The expression proportion of Nrf2 in IBA1 positive cells increased gradually, reached its peak at 5-7 d post-injury, and then decreased. Conclusion The expression of Nrf2 in different cells involves in the biological function of different cells post-injury, and the dynamic expression of single cells has a time-dependent pattern. This may provide a new reference index for the wound age estimation of brain contusion in human.