LC-MS-Based Lipidomics and Automated Identification of Lipids Using the LipidBlast In-Silico MS/MS Library
LC-MS-Based Lipidomics and Automated Identification of Lipids Using the LipidBlast In-Silico MS/MS Library
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DOI：
10.1007/978-1-4939-6996-8_14
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Fiehn, Oliver
中科院分区：
文献类型：
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作者：
Cajka, Tomas;Fiehn, Oliver
本协议描述了分析，特别是鉴定，血浆脂质。使用甲基叔丁基醚（MTBE）、甲醇和水提取血浆脂质，然后使用反相液相色谱耦合四极杆/飞行时间质谱（RPLC-QTOFMS）在MS/MS模式下对分离的脂质进行分离和数据采集。为了进行脂质鉴定，将获取的MS/MS光谱转换为通用格式（MGF），然后使用计算机MS/MS库LipidBlast进行文库搜索。使用这种方法，脂类、碳链长度和脂肪酸组分的不饱和程度被注释。
This protocol describes the analysis, specifically the identification, of blood plasma lipids. Plasma lipids are extracted using methyl tert-butyl ether (MTBE), methanol, and water followed by separation and data acquisition of isolated lipids using reversed-phase liquid chromatography coupled to quadrupole/time-of-flight mass spectrometry (RPLC-QTOFMS) operated in MS/MS mode. For lipid identification, acquired MS/MS spectra are converted to the mascot generic format (MGF) followed by library search using the in-silico MS/MS library LipidBlast. Using this approach, lipid classes, carbon-chain lengths, and degree of unsaturation of fatty-acid components are annotated.