A novel Giraffidae-specific interspersed repeat with a microsatellite, originally found in an intron of a ruminant paralogous p97bcnt gene.
A novel Giraffidae-specific interspersed repeat with a microsatellite, originally found in an intron of a ruminant paralogous p97bcnt gene.
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一种新颖的长颈鹿科特异性散布重复序列，带有微卫星，最初发现于反刍动物旁系同源 p97bcnt 基因的内含子中。
DOI：
10.1016/j.gene.2004.07.016
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发表时间：
2004
期刊：
影响因子：
3.5
通讯作者：
S. Iwashita
中科院分区：
文献类型：
--
作者：
K. Hon;Sadao Ueno;H. Endo;H. Nishimura;T. Igarashi;L. David;S. Iwashita
反刍动物特异性p97 bcnt基因（bcntp 97）是一个旁系同源基因，包括一个来自逆转录转座因子1（RTE-1）的区域。该区域包含编码p97 bcnt蛋白中间325个氨基酸的外显子（RTE-1外显子）。为了了解bcntp 97基因是如何进化的，我们研究了它在几种反刍动物中的组织。我们在长颈鹿bcntp 97的RTE-1外显子的5′内含子中发现了一个700 bp的插入片段。在牛和西卡鹿的相应区域中，该插入片段缺失。此外，插入物的序列散布在长颈鹿的基因组中而不是牛的基因组中，并且还包含（GA）n微卫星。一个高度同源的插入窝藏显着不同的（GA）n微卫星检测到在相应区域的霍加bcntp 97。因此，散布片段与（GA）n微卫星可能作为一个标记，跟踪重复基因如何在一个家庭特异性的方式演变。
The ruminant-specific p97bcnt gene (bcntp97) is a paralogous gene that includes a region derived from a retrotransposable element 1 (RTE-1). The region comprises an exon (RTE-1 exon) encoding 325 amino acids in the middle of the p97bcnt protein. To understand how the bcntp97paralog evolved, we examined its organization in several ruminants. We found a 700-base pair (bp) insert in the 5′ intron of the RTE-1 exon in giraffe bcntp97. This insert is missing in the corresponding regions of bovine and sika deer. Furthermore, the sequence of the insert is interspersed in the genome of giraffe but not bovine and also contains a (GA)n microsatellite. A highly homologous insert harboring significantly different (GA)n microsatellite was detected in the corresponding region of okapi bcntp97. Therefore, the interspersed fragments with (GA)n microsatellite might serve as a marker for tracking how duplicated genes evolve in a family-specific manner.