High Vitreous Concentration of IL-6 and IL-8, but Not of Adhesion Molecules in Relation to Plasma Concentrations in Proliferative Diabetic Retinopathy
High Vitreous Concentration of IL-6 and IL-8, but Not of Adhesion Molecules in Relation to Plasma Concentrations in Proliferative Diabetic Retinopathy
复制标题
DOI：
10.1159/000342977
复制
发表时间：
2013-01-01
影响因子：
2.1
通讯作者：
Liinamaa, M. J.
中科院分区：
文献类型：
--
作者：
Koskela, U. E.;Kuusisto, S. M.;Liinamaa, M. J.
背景/目的：炎症标志物已在增殖性糖尿病视网膜病变（PDR）中观察到。我们评估了PDR和非糖尿病对照组玻璃体中粘附分子和细胞因子的浓度以及血浆浓度，以区分局部炎症和血视网膜屏障的破坏。方法：对38例PDR患者和16例对照组黄斑裂孔或视网膜前膜患者行玻璃体切割术。维生素和血浆可溶性粘附分子[sE-选择素、细胞间粘附分子（sICAM）-1和-3、血小板-内皮细胞粘附分子（sPECAM）-1、sP-选择素、血管细胞粘附分子（sVCAM）-1]和细胞因子[白细胞介素（IL）-1 β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-12（p70）、肿瘤坏死因子-α和-β、γ-干扰素]通过多重测定检测。结果：PDR中玻璃体中IL-6和IL-8的水平比血浆中高26倍（p = 0.001）和6倍（p = 0.001）。PDR患者的Vitamin IL-10（p = 0.004）、sPECAM-1、sE-选择素、sICAM-1和sVCAM-1均高于对照组（均为p = 0.001）。PDR组玻璃体中粘附分子浓度低于血浆中粘附分子浓度的10%。玻璃体中的IL-10低于血浆（3.0对12.8 pg/ml，p = 0.007），并且PDR中的玻璃体IL-10/IL-8比率显著低于对照（0.10对0.55 pg/ml，p = 0.003）。结论：PDR患者玻璃体中IL-6和IL-8水平升高，而血浆中IL-6和IL-8水平无明显变化，提示PDR患者局部炎症反应较全身炎症反应强。此外，玻璃体中的炎症和抗炎细胞因子之间存在失衡。版权所有（c）2012 S. Karger AG，巴塞尔
Background/Aim: Inflammatory markers have been observed in proliferative diabetic retinopathy (PDR). We assessed vitreous concentrations of adhesion molecules and cytokines in PDR and non-diabetic controls and plasma concentrations to differentiate local inflammation from the breakdown of the blood-retina barrier. Methods: 38 patients with PDR and 16 controls with macular hole or epiretinal membrane underwent vitrectomy. Vitreous and plasma soluble adhesion molecules [sE-selectin, intercellular adhesion molecule (sICAM)-1 and -3, platelet-endothelial cell adhesion molecule (sPECAM)-1, sP-selectin, vascular cell adhesion molecule (sVCAM)-1] and cytokines [interleukin (IL)-1 beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), tumour necrosis factor-alpha and -beta, gamma-interferon] were detected by the multiplex assay. Results: Levels of IL-6 and IL-8 were 26-fold (p = 0.001) and 6-fold higher (p = 0.001) in vitreous than in plasma in PDR. Vitreous IL-10 (p = 0.004), sPECAM-1, sE-selectin, sICAM-1 and sVCAM-1 were higher in PDR than controls (p = 0.001 for all). Adhesion molecule concentrations in vitreous in PDR were less than 10% of those in plasma. IL-10 was lower in vitreous than plasma (3.0 vs. 12.8 pg/ml, p = 0.007), and the vitreous IL-10/IL-8 ratio was significantly lower in PDR than in controls (0.10 vs. 0.55 pg/ml, p = 0.003). Conclusion: The elevated IL-6 and IL-8 levels in vitreous, but not in plasma, are evidence favouring local over systemic inflammation in PDR. Furthermore, there was imbalance between inflammatory and anti-inflammatory cytokines in the vitreous. Copyright (c) 2012 S. Karger AG, Basel