The formation of stable E rosettes after neuraminidase treatment of either human peripheral blood lymphocytes or of sheep red blood cells.
The formation of stable E rosettes after neuraminidase treatment of either human peripheral blood lymphocytes or of sheep red blood cells.
复制标题
神经氨酸酶处理人外周血淋巴细胞或绵羊红细胞后形成稳定的 E 玫瑰花结。
DOI：
10.4049/jimmunol.112.5.1628
复制
发表时间：
1974
影响因子：
4.4
通讯作者：
M. Schlesinger
中科院分区：
文献类型：
--
作者：
U. Galili;M. Schlesinger
人外周血淋巴细胞（PBL）与羊红细胞（SRBC）形成E型莲座，在37°C孵卵后容易解体，摇晃容易破坏。研究发现，PBL或SRBC的神经氨酸酶处理增加了稳定莲座的比例，可以抵抗37°C长时间孵育和剧烈摇动。形成稳定莲座的比例与使用的神经氨酸酶浓度有关。SRBC必须暴露于比PBL更高浓度的神经氨酸酶才能产生稳定的莲座。PBL与吸收兔抗血清（RAHT）孵育人胸腺，完全阻止稳定或规则E花环的形成。兔抗血清对人骨髓和脑无明显影响。经神经氨酸酶处理的SRBC或PBL抑制玫瑰花形成所需的RAHT浓度高于未经处理的细胞抑制玫瑰花形成所需的RAHT浓度。这表明，神经氨酸酶不会“揭开”SRBC或PBL上的受体位点，而是减少细胞的表面电荷，从而使暴露在PBL膜上的受体与SRBC上的位点之间能够更有效地相互作用。
Human peripheral blood lymphocytes (PBL) form E rosettes with sheep red blood cells (SRBC) which tend to disintegrate upon incubation at 37°C and which can be disrupted easily by shaking. Neuraminidase treatment of either PBL or SRBC was found to increase the proportion of stable rosettes resistant to prolonged incubation at 37°C and to vigorous shaking. The proportion of stable rosettes formed was related to the concentration of neuraminidase used. SRBC had to be exposed to higher concentrations of neuraminidase than PBL for the production of stable rosettes. Incubation of PBL with absorbed rabbit antiserum to human thymus (RAHT) completely prevented the formation of either stable or regular E rosettes. Rabbit antisera to human bone marrow or brain had no such effect. The concentration of RAHT required for the inhibition of rosette formation by neuraminidase-treated SRBC or PBL was higher than that required for the inhibition of rosette formation by untreated cells. It is suggested that neuraminidase does not “unmask” receptor sites on either SRBC or PBL, but rather reduces the electric surface charge of the cells, thereby enabling a more effective interaction between receptors exposed on the membrane of PBL and sites on SRBC.