The Bacteroides fragilis P20 scavengase homolog is important in the oxidative stress response but is not controlled by OxyR.
The Bacteroides fragilis P20 scavengase homolog is important in the oxidative stress response but is not controlled by OxyR.
复制标题
脆弱拟杆菌 P20 清除酶同系物在氧化应激反应中很重要，但不受 OxyR 控制。
DOI：
10.1111/j.1574-6968.2006.00353.x
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发表时间：
2006
影响因子：
2.1
通讯作者：
JeffreySmith,C
中科院分区：
文献类型：
--
作者：
Sund,ChristianJ;GregWells,William;JeffreySmith,C
The oxidative stress response of obligate anaerobe,Bacteroides fragilis, is partially controlled by the redox regulator OxyR but anoxyRnull mutant maintains a high level of aerotolerance. Studies using two-dimensional polyacrylamide gel electrophoresis showed that a thiol peroxidase-scavengase, Tps, was induced during oxygen exposure of anoxyRmutant. Tps is similar to ‘atypical 2-cysteine peroxidases’ such as scavengase p20 and it demonstrated catalytic activity againstt-butyl hydroperoxide and H2O2. A second gene,oim, encoding a putative membrane protein, was divergently transcribed fromtps. Transcriptional analysis indicated thattpsandoimwere coordinately regulated by oxygen induction via an OxyR-independent mechanism. H2O2was a less potent inducer than oxygen exposure and in anoxyRmutant the mRNA levels were slightly reduced compared with the wild type. A null mutant oftpshad increased sensitivity to killing byt-butyl hydroperoxide and oxygen but anoimmutant was similar to wild type. These data indicate that Tps is important for protection against some forms of oxidative stress.
