Repeatability of brown adipose tissue measurements on FDG PET/CT following a simple cooling procedure for BAT activation
Repeatability of brown adipose tissue measurements on FDG PET/CT following a simple cooling procedure for BAT activation
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DOI：
10.1371/journal.pone.0214765
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发表时间：
2019-04-17
期刊：
影响因子：
3.7
通讯作者：
Wahl, Richard
中科院分区：
文献类型：
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作者：
Crandall, John;Gajwani, Prateek;Wahl, Richard
棕色脂肪组织（BAT）存在于相当数量的成年人中，并且可以通过暴露于寒冷而激活。测量活性BAT的存在、活性和体积对于确定旨在激活BAT的潜在治疗的功效是期望的。在受控条件下，18F-氟脱氧葡萄糖（FDG）正电子发射断层扫描（PET）测量BAT存在、活性和体积的可重复性尚未得到广泛研究。11名女性志愿者接受了双基线FDG PET成像，该成像是在旨在激活棕色脂肪的简单区域性冷干预后进行的。冷干预包括穿着轻薄的参与者间歇性地将脚放在冰块上，同时坐在凉爽的房间里。在两周的目标时间内，在相同条件下进行重复研究。获得FDG扫描，并根据布朗脂肪成像研究报告标准（BARCIST）1.0确定针对瘦体重调整的最大标准化摄取值（SULmax）、CT亨氏单位（HU）、BAT代谢体积（BMV）和总BAT糖酵解（TBG）。林的一致性相关性（CCC）为0.80的BMV之间的测试和重新测试成像。会话间BAT SULmax显著相关（r = 0.54; p < 0.05）。第1阶段的平均SULmax为4.92 +/- 4.49 g/mL，与第2阶段的平均SULmax为7.19 +/- 7.34 g/mL（p = 0.16）无显著差异。BAT SULmax与BMV在测试和再测试研究中高度相关（r >= 0.96，p < 0.001）。使用简化的冰块冷却方法，BAT在大多数（9/11）年轻，瘦女性参与者中被激活。BAT SUL和BMV的定量评估在测试和重新测试成像之间没有实质性差异，但个体BMV可能差异很大。会话内BMV和SULmax强烈相关。在用FDG进行的重测中BAT活性和体积估计值的变异性应告知在量化BAT生理学的研究中样本量的选择，并支持该组织的动态代谢特征。一种更复杂的冷却方法可能会减少重复测试的最佳可得技术研究中的变化。
Brown Adipose Tissue (BAT) is present in a significant number of adult humans and can be activated by exposure to cold. Measurement of active BAT presence, activity, and volume are desirable for determining the efficacy of potential treatments intended to activate BAT. The repeatability of 18F-fluorodeoxyglucose (FDG) positron emission tomography (PET) measurements of BAT presence, activity, and volume under controlled conditions has not been extensively studied. Eleven female volunteers underwent double baseline FDG PET imaging performed following a simple, regional cold intervention intended to activate brown fat. The cold intervention involved the lightly-clothed participants intermittently placing their feet on a block of ice while sitting in a cooled room. A repeat study was performed under the same conditions within a target of two weeks. FDG scans were obtained and maximum standardized uptake value adjusted for lean body mass (SULmax), CT Hounsfield units (HU), BAT metabolic volume (BMV), and total BAT glycolysis (TBG) were determined according to the Brown Adipose Reporting Criteria in Imaging STudies (BARCIST) 1.0. A Lin's concordance correlation (CCC) of 0.80 was found for BMV between test and retest imaging. Intersession BAT SULmax was significantly correlated (r = 0.54; p < 0.05). The session #1 mean SULmax of 4.92 +/- 4.49 g/mL was not significantly different from that of session #2 with a mean SULmax of 7.19 +/- 7.34 g/mL (p = 0.16). BAT SULmax was highly correlated with BMV in test and retest studies (r >= 0.96, p < 0.001). Using a simplified iceblock cooling method, BAT was activated in the majority (9/11) of a group of young, lean female participants. Quantitative assessments of BAT SUL and BMV were not substantially different between test and retest imaging, but individual BMV could vary considerably. Intra-session BMV and SULmax were strongly correlated. The variability in estimates of BAT activity and volume on test-retest with FDG should inform sample size choice in studies quantifying BAT physiology and support the dynamic metabolic characteristics of this tissue. A more sophisticated cooling method potentially may reduce variations in test-retest BAT studies.