MANGANESE STATUS, GUT ENDOGENOUS LOSSES OF MANGANESE, AND ANTIOXIDANT ENZYME-ACTIVITY IN RATS FED VARYING LEVELS OF MANGANESE AND FAT
MANGANESE STATUS, GUT ENDOGENOUS LOSSES OF MANGANESE, AND ANTIOXIDANT ENZYME-ACTIVITY IN RATS FED VARYING LEVELS OF MANGANESE AND FAT
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DOI：
10.1007/bf02990485
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发表时间：
1994-07-01
影响因子：
3.9
通讯作者：
GREGER, JL
中科院分区：
文献类型：
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作者：
MALECKI, EA;HUTTNER, DL;GREGER, JL
我们假设，饲喂高水平和中等水平多不饱和脂肪的缺锰动物要么会表现出氧化应激增加的证据，要么会经历抗氧化酶的补偿性变化和/或锰利用率的变化，从而导致内源性肠道锰损失减少。大鼠（研究 1 中为雌性，研究 2 中为雄性，n = 8/处理）的饮食中含有按重量计 5% 或 20% 的玉米油以及 0.01 或 1.5 μmol 锰/g 饮食。在研究 2 中，将与白蛋白复合的 Mn-54 注射到门静脉中，以评估肠道内源性锰损失。缺锰大鼠： 1．肝脏、胫骨、肾脏、脾脏和胰腺的锰浓度比锰充足大鼠低30-50%；2．通过将锰的内源性粪便损失减少约 70 倍来保护锰；3．心脏锰超氧化物歧化酶（MnSOD）活性较低；和4。铜锌超氧化物歧化酶 (CuZnSOD) 和过氧化氢酶的活性仅经历两次较小的代偿性变化。在喂食高脂肪饮食的大鼠中，肠道内源性锰损失往往占吸收锰的较小比例；否则，脂肪摄入对组织锰浓度影响不大。
We hypothesized that manganese deficient animals fed high vs moderate levels of polyunsaturated fat would either manifest evidence of increased oxidative stress or would experience compensatory changes in antioxidant enzymes and/or shifts in manganese utilization that result in decreased endogenous gut manganese losses. Rats (females in Study 1, males in Study 2, n = 8/treatment) were fed diets that contained 5 or 20% corn oil by weight and either 0.01 or 1.5 mu mol manganese/g diet. In study 2, Mn-54 complexed to albumin was injected into the portal vein to assess gut endogenous losses of manganese. The manganese deficient rats:1. Had 30-50% lower liver, tibia, kidney, spleen, and pancreas manganese concentrations than manganese adequate rats;2. Conserved manganese through approximate to 70-fold reductions in endogenous fecal losses of manganese;3. Had lower heart manganese superoxide dismutase (MnSOD) activity; and4. Experienced only two minor compensatory changes in the activity of copper-zinc superoxide dismutase (CuZnSOD) and catalase.Gut endogenous losses of manganese tended to account for a smaller proportion of absorbed manganese in rats fed high-fat diets; otherwise fat intake had few effects on tissue manganese concentrations.