Short-Term Moderately Elevated Intraocular Pressure Is Associated With Elevated Scotopic Electroretinogram Responses.
Short-Term Moderately Elevated Intraocular Pressure Is Associated With Elevated Scotopic Electroretinogram Responses.
复制标题
DOI：
10.1167/iovs.15-18770
复制
发表时间：
2016-04-01
影响因子：
4.4
通讯作者：
Joos KM
中科院分区：
文献类型：
--
作者：
Choh V;Gurdita A;Tan B;Prasad RC;Bizheva K;Joos KM
中度升高的眼内压（IOP）是开角型青光眼的危险因素。一些患者患有青光眼，尽管临床测量的IOP正常。IOP的波动可能具有重要作用，因为IOP在睡眠和倒置活动期间较高。随着时间的推移，大鼠中IOP的受控短暂升高导致视神经结构变化，这与在青光眼的恒定慢性模型中观察到的早期变化相似。由于早期干预可减少青光眼进展，因此进行本研究以确定在中度升高的IOP期间，是否可以通过非侵入性电生理和光学成像测试检测到视网膜的早期生理变化。将Sprague-Dawley大鼠的一只眼的眼内压升高至中等高水平（35 mm Hg），而另一只（对照）眼未接受治疗。一组大鼠进行暗视阈值反应（STR）和视网膜电图（ERG）测试，而另外3组进行光学相干断层扫描（OCT）成像，蛋白质印迹，或组织学评价。与IOP升高前的值相比，以及与未治疗的对侧眼相比，IOP中度升高的眼的STR和ERG反应的幅度增强。在IOP升高期间也发生视神经的结构变化。虽然众所周知缺血性IOP升高会在整体上降低暗视ERG的成分，但大鼠中急性升高至未治疗青光眼患者中经常观察到的水平会导致这些参数增加。进一步探讨这些现象可能有助于更好地理解在波动或慢性高眼压期间介导早期视网膜变化的机制。
Moderately elevated intraocular pressure (IOP) is a risk factor for open-angle glaucoma. Some patients suffer glaucoma despite clinically measured normal IOPs. Fluctuations in IOP may have a significant role since IOPs are higher during sleep and inversion activities. Controlled transient elevations of IOPs in rats over time lead to optic nerve structural changes that are similar to the early changes observed in constant chronic models of glaucoma. Because early intervention decreases glaucoma progression, this study was done to determine if early physiological changes to the retina could be detected with noninvasive electrophysiological and optical imaging tests during moderately elevated IOP. Intraocular pressures were raised to moderately high levels (35 mm Hg) in one eye of Sprague-Dawley rats while the other (control) eye was untreated. One group of rats underwent scotopic threshold response (STR) and electroretinogram (ERG) testing, while another 3 groups underwent optical coherence tomography (OCT) imaging, Western blot, or histologic evaluation. The amplitudes of the STR and ERG responses in eyes with moderately elevated IOPs were enhanced compared to the values before IOP elevation, and compared to untreated contralateral eyes. Structural changes to the optic nerve also occurred during IOP elevation. Although ischemic IOP elevations are well-known to globally reduce components of the scotopic ERG, acute elevation in rats to levels often observed in untreated glaucoma patients caused an increase in these parameters. Further exploration of these phenomena may be helpful in better understanding the mechanisms mediating early retinal changes during fluctuating or chronically elevated IOP.