The effective bioengineering method of implantation decellularized renal extracellular matrix scaffolds.
The effective bioengineering method of implantation decellularized renal extracellular matrix scaffolds.
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植入脱细胞肾细胞外基质支架的有效生物工程方法
DOI：
10.18632/oncotarget.5304
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发表时间：
2015-11-03
期刊：
影响因子：
--
通讯作者：
Liu Y
中科院分区：
文献类型：
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作者：
Guan Y;Liu S;Sun C;Cheng G;Kong F;Luan Y;Xie X;Zhao S;Zhang D;Wang J;Li K;Liu Y
终末期肾病(ESRD)是一种进行性肾功能丧失，具有较高的发病率和死亡率。可移植的器官很难获得，而且有很高的受体免疫排斥反应风险。我们打算建立一种更有效和更快的方法来脱细胞和再细胞的肾支架移植和再生。我们用0.5%十二烷基硫酸钠(十二烷基硫酸钠)对大鼠肾脏进行脱细胞处理，成功地制备了肾支架，同时仍保留了细胞外基质(ECM)的3D结构、完整的血管树和生化成分。我们用小鼠胚胎干细胞(ES)对肾支架进行再细胞化，然后在肾小球、血管和肾小管结构中填充和增殖。体内植入后，这些再细胞支架容易再灌流，耐受血压，并产生尿液，没有血液渗漏。我们的方法可以成功地脱细胞和再细胞大鼠肾脏，以生产功能肾ECM支架。这些支架保持了它们的基本成分，保留了完整的血管系统，并显示出肾脏再生的前景。
End stage renal disease (ESRD) is a progressive loss of kidney function with a high rate of morbidity and mortality. Transplantable organs are hard to come by and hold a high risk of recipient immune rejection. We intended to establish a more effective and faster method to decellularize and recellularize the kidney scaffold for transplant and regeneration. We successfully produced renal scaffolds by decellularizing rat kidneys with 0.5% sodium dodecyl sulfate (SDS), while still preserving the extracellular matrix (ECM) 3D architecture, an intact vascular tree and biochemical components. We recellularized the kidney scaffolds with mouse embryonic stem (ES) cells that then populated and proliferated within the glomerular, vascular, and tubular structures. After in vivo implantation, these recellularized scaffolds were easily reperfused, tolerated blood pressure and produced urine with no blood leakage. Our methods can successfully decellularize and recellularize rat kidneys to produce functional renal ECM scaffolds. These scaffolds maintain their basic components, retain intact vasculature and show promise for kidney regeneration.