Combinatorial synthesis of peptide arrays onto a microchip
Combinatorial synthesis of peptide arrays onto a microchip
复制标题
DOI：
10.1126/science.1149751
复制
发表时间：
2007-12-21
期刊：
影响因子：
56.9
通讯作者：
Breitling, Frank
中科院分区：
文献类型：
--
作者：
Beyer, Mario;Nesterov, Alexander;Breitling, Frank
阵列有望通过平行筛选结合伴侣来推进生物学。我们展示了在微芯片上每平方厘米40，000个肽点的组合原位合成。我们的变体梅里菲尔德合成将活化的氨基酸作为单体固定在颗粒内，这些颗粒被芯片的每个像素电极上产生的电场连续吸引。随着所有不同的氨基酸被寻址，颗粒立即熔化以启动偶联。重复偶联循环应允许将整个蛋白质组翻译成重叠肽阵列，其可用于蛋白质组研究和抗体分析。
Arrays promise to advance biology through parallel screening for binding partners. We show the combinatorial in situ synthesis of 40,000 peptide spots per square centimeter on a microchip. Our variant Merrifield synthesis immobilizes activated amino acids as monomers within particles, which are successively attracted by electric fields generated on each pixel electrode of the chip. With all different amino acids addressed, particles are melted at once to initiate coupling. Repetitive coupling cycles should allow for the translation of whole proteomes into arrays of overlapping peptides that could be used for proteome research and antibody profiling.