Microanatomical changes and biomolecular expression at the PDL-entheses during experimental tooth movement.
Microanatomical changes and biomolecular expression at the PDL-entheses during experimental tooth movement.
复制标题
实验性牙齿移动过程中 PDL 附着点的显微解剖变化和生物分子表达。
DOI：
10.1111/jre.12625
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发表时间：
2019
影响因子：
3.5
通讯作者：
Ho,SunitaP
中科院分区：
文献类型：
--
作者：
Yang,Lynn;Kang,Misun;He,Rui;Meng,Bo;Pal,Arvin;Chen,Ling;Jheon,AndrewH;Ho,SunitaP
本研究的新方面是将生物分子表达在机械活化的牙周复合体内加宽和变窄的牙周膜（PDL）空间中的共定位联系起来。PDL是独特的，因为它是唯一既有神经支配又有血管形成的韧带。使用弹性间隔器对6周龄雄性C57 BL/6小鼠（N = 5）的上颌磨牙进行2周的实验性平移。侧牙作为对照。原位小鼠牙周复合体的力学测试和使用X射线微计算机断层扫描（micro-XCT）的成像显示了PDL血管（BV）内的变形。实验性牙齿移动（ETM）后，PDL-骨和PDL-牙骨质附着体在PDL-间隙加宽和变窄处显示了osterix（OSX）、骨唾液蛋白（BSP）、分化簇146（CD 146）和蛋白基因产物9.5（PGP 9.5），表明这些部位的主动重塑。PGP 9.5阳性神经束（NB）与多核细胞（MC）、Howship吸收陷窝和CD 146阳性BV共定位。神经和MC之间的关联进行了补充，通过进行扫描透射电子显微镜观察四氧化锇染色的NB与MC的超微结构的接近。NB与BV以及NB与MC的空间相关性为NB启动骨细胞活性的合理共激活提供了见解。尝试恢复承重复合体的PDL-空间，特别是PDL-附着点处的矿物吸收。通过关联光学和电子显微镜照片绘制解剖学特异性结构元件及其与再生分子的关联，为使用这些细胞外基质分子作为与牙齿移动相关的药理学干预的合理靶点提供了见解。在组织再生领域内，负荷的调节可以将自然发生的矿物质形成逆转为实验诱导的再吸收，并且将自然发生的矿物质再吸收逆转为在附着点处实验诱导的形成，以允许牙齿平移。
The novel aspect of this study was to contextualize the co‐localization of biomolecular expression in widened and narrowed periodontal ligament (PDL)‐space within a mechanically activated periodontal complex. The PDL is unique as it is the only ligament with both innervation and vascularization. Maxillary molars in 6‐week‐old male C57BL/6 mice (N = 5) were experimentally translated for 2 weeks using an elastic spacer. Contralateral teeth were used as controls. Mechanical testing of the periodontal complex of a mouse in situ and imaging using X‐ray micro‐computed tomography (micro‐XCT) illustrated deformations within blood vessels (BV) of the PDL. PDL‐bone and PDL‐cementum entheses at the widened and narrowed PDL‐spaces following experimental tooth movement (ETM) illustrated osterix (OSX), bone sialoprotein (BSP), cluster of differentiation 146 (CD146), and protein gene product 9.5 (PGP9.5), indicating active remodeling at these sites. PGP9.5 positive nerve bundles (NBs) were co‐localized with multinucleated cells (MCs), Howship's resorption lacunae, and CD146 positive BVs. Association between nerves and MC was complemented by visualizing the proximity of osmium tetroxide stained NBs with the ultrastructure of MCs by performing scanning transmission electron microscopy. Spatial association of NB with BV, and NB with MC, provided insights into the plausible co‐activation of NBs to initiate osteoclastic activity. Resorption of mineral occurred as an attempt to restore PDL‐space of the load‐bearing complex, specifically at the PDL‐entheses. Mapping of anatomy‐specific structural elements and their association with regenerative molecules by correlating light and electron micrographs provided insights into the use of these extracellular matrix molecules as plausible targets for pharmacological interventions related to tooth movement. Within the realm of tissue regeneration, modulation of load can reverse naturally occurring mineral formation to experimentally induced resorption, and naturally occurring mineral resorption to experimentally induced formation at the enthesial sites to permit tooth translation.