Analyzing notochord segmentation and intervertebral disc formation using the twhh:gfp transgenic zebrafish model
Analyzing notochord segmentation and intervertebral disc formation using the twhh:gfp transgenic zebrafish model
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DOI：
10.1007/s11248-009-9259-y
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发表时间：
2009-10-01
影响因子：
3
通讯作者：
Du, Shao Jun
中科院分区：
文献类型：
--
作者：
Haga, Yutaka;Dominique, Vincent J., III;Du, Shao Jun
为了表征活体动物脊椎分割和椎间盘形成的过程，我们分析了虎刺刺猬（twhh）：绿色荧光蛋白（gfp）和音刺刺猬（shh）：gfp转基因斑马鱼模型，显示脊索特异性GFP表达。我们发现它们在晚期仔鱼和成年斑马鱼的椎间盘中表现出不同的表达模式。在twhh：gfp转基因鱼的椎间盘中检测到GFP表达的分段模式。相反，在shh：gfp转基因鱼的椎间盘中发现很少GFP表达。外源性视黄酸（RA）是一种对正常发育有致畸作用的因子，用RA处理转twhh：gfp基因斑马鱼幼鱼，可导致脊索分节中断，并形成过大的椎骨。组织学分析显示，过大的椎骨可能是由于椎骨融合。这些研究表明，twhh：gfp转基因斑马鱼是一个有用的模型，研究脊椎分割和椎间盘形成，此外，RA信号可能在这个过程中发挥作用。
To characterize the process of vertebral segmentation and disc formation in living animals, we analyzed tiggy-winkle hedgehog (twhh):green fluorescent protein (gfp) and sonic hedgehog (shh):gfp transgenic zebrafish models that display notochord-specific GFP expression. We found that they showed distinct patterns of expression in the intervertebral discs of late stage fish larvae and adult zebrafish. A segmented pattern of GFP expression was detected in the intervertebral disc of twhh:gfp transgenic fish. In contrast, little GFP expression was found in the intervertebral disc of shh:gfp transgenic fish. Treating twhh:gfp transgenic zebrafish larvae with exogenous retinoic acid (RA), a teratogenic factor on normal development, resulted in disruption of notochord segmentation and formation of oversized vertebrae. Histological analysis revealed that the oversized vertebrae are likely due to vertebral fusion. These studies demonstrate that the twhh:gfp transgenic zebrafish is a useful model for studying vertebral segmentation and disc formation, and moreover, that RA signaling may play a role in this process.