High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
High-precision microbeam radiotherapy reveals testicular tissue-sparing effects for male fertility preservation
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DOI：
10.1038/s41598-019-48772-3
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发表时间：
2019-10-01
影响因子：
4.6
通讯作者：
Prise, Kevin M.
中科院分区：
文献类型：
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作者：
Fukunaga, Hisanori;Kaminaga, Kiichi;Prise, Kevin M.
微束放射治疗(MRT)是基于同步辐射X射线微束在微尺度上的空间分割。尽管对非均匀辐射场的组织节约效应(TSE)早在一个多世纪前就已被认识到，但MRT在睾丸的TSE及其对预防男性生育的临床意义仍有待确定。在这项研究中，使用MRT技术和独特的体外睾丸器官培养相结合，我们首次展示了MRT介导的TSE用于保存精子发生。此外，我们的高精度微束分析表明，非照射的生殖干细胞在照射后的睾丸组织中的存活和潜在的迁移步骤将是有效的TSE用于精子发生所必需的。我们的研究结果表明，睾丸在微尺度水平上的辐射剂量分布对于向肿瘤提供高剂量辐射，同时仍保持男性生育能力具有临床重要性。
Microbeam radiotherapy (MRT) is based on a spatial fractionation of synchrotron X-ray microbeams at the microscale level. Although the tissue-sparing effect (TSE) in response to non-uniform radiation fields was recognized more than one century ago, the TSE of MRT in the testes and its clinical importance for preventing male fertility remain to be determined. In this study, using the combination of MRT techniques and a unique ex vivo testes organ culture, we show, for the first time, the MRT-mediated TSE for the preservation of spermatogenesis. Furthermore, our high-precision microbeam analysis revealed that the survival and potential migration steps of the non-irradiated germ stem cells in the irradiated testes tissue would be needed for the effective TSE for spermatogenesis. Our findings indicated the distribution of dose irradiated in the testes at the microscale level is of clinical importance for delivering high doses of radiation to the tumor, while still preserving male fertility.