Nitrosophilus kaiyonis sp. nov., a hydrogen-, sulfur- and thiosulfate-oxidizing chemolithoautotroph within “Campylobacteria” isolated from a deep-sea hydrothermal vent in the Mid-Okinawa Trough
Nitrosophilus kaiyonis sp. nov., a hydrogen-, sulfur- and thiosulfate-oxidizing chemolithoautotroph within “Campylobacteria” isolated from a deep-sea hydrothermal vent in the Mid-Okinawa Trough
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DOI：
10.1007/s00203-022-03350-6
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发表时间：
2022-12
影响因子：
2.8
通讯作者：
soichi Fukazawa;Sayaka Mino;Jiro Tsuchiya;S. Nakagawa;K. Takai;T. Sawabe
中科院分区：
文献类型：
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作者：
soichi Fukazawa;Sayaka Mino;Jiro Tsuchiya;S. Nakagawa;K. Takai;T. Sawabe
从冲绳中部海槽伊平屋北场的烟囱结构中分离出一种新细菌，菌株 MOT50T。这些细胞是具有单极鞭毛的活动短杆。在 40 至 65℃（最佳 52℃）、pH 值 5.0 至 7.1（最佳 pH 6.1）以及 2.0–4.0% NaCl（最佳 2.5%）存在下观察到生长。分离物利用分子氢、硫代硫酸盐或元素硫作为唯一的电子供体。硫代硫酸盐、元素硫、硝酸盐和分子氧被用作唯一的电子受体。需要铵作为氮源。硫代硫酸盐、元素硫、硫酸盐或亚硫酸盐充当生长的硫源。基因组DNA的G + C含量为28.9%。基于16S rRNA基因序列的系统发育分析表明，菌株MOT50T属于“弯曲菌”纲Nitrosophilus属，其近亲为Nitrosophilus labiiHRV44T(97.20%)。根据系统发育、生理和分子特征，该生物体代表了亚硝化菌属（Nitrosophilus kaiyonissp）中的一个新物种。十一月模式菌株为MOT50T(= JCM 39187T= KCTC 25251T)。
A novel bacterium, strain MOT50T, was isolated from the chimney structure at the Iheya North field in the Mid-Okinawa Trough. The cells were motile short rods with a single polar flagellum. Growth was observed between 40 and 65 ℃ (optimum, 52 ℃), at pH values between 5.0 and 7.1 (optimum, pH 6.1) and in the presence of 2.0–4.0% NaCl (optimum, 2.5%). The isolates utilized molecular hydrogen, thiosulfate, or elemental sulfur as the sole electron donor. Thiosulfate, elemental sulfur, nitrate, and molecular oxygen are utilized as the sole electron acceptor. Ammonium is required as a nitrogen source. Thiosulfate, elemental sulfur, sulfate, or sulfite serves as a sulfur source for growth. The G + C content of the genomic DNA was 28.9%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain MOT50Tbelonged to the genusNitrosophilusof the class “Campylobacteria”, and its closest relative wasNitrosophilus labiiHRV44T(97.20%). On the basis of the phylogenetic, physiological, and molecular characteristics, it is proposed that the organism represents a novel species within the genusNitrosophilus,Nitrosophilus kaiyonissp. nov. The type strain is MOT50T(= JCM 39187T= KCTC 25251T).