Resonant Raman detection of macular pigment levels in the living human retina
Resonant Raman detection of macular pigment levels in the living human retina
复制标题
DOI：
10.1364/ol.26.000202
复制
发表时间：
2001-02-15
期刊：
影响因子：
3.6
通讯作者：
Bernstein, PS
中科院分区：
文献类型：
--
作者：
Ermakov, IV;McClane, RW;Bernstein, PS
我们已经使用共振拉曼散射作为一种新的，非侵入性的体内光学技术来测量的浓度黄斑类胡萝卜素色素在活的人视网膜。使用后向散射几何和共振分子激发在可见光下，我们测量的拉曼峰，起源于单，双键伸缩振动的π共轭分子的碳骨架。拉曼信号与类胡萝卜素含量成线性比例，而所需的激光激发远低于黄斑暴露的安全限值。该拉曼技术是客观和定量的，并可能导致一种新的方法，用于快速筛选类胡萝卜素色素水平在大的人群中，是在风险的视力丧失的年龄相关性黄斑变性，失明的主要原因，在美国。(C)2001美国光学学会OCIS编码器;：170.3890、170.4470、160.4890、290.5860、300.6450、330.4300。
We have used resonant Raman scattering as a novel, noninvasive in vivo optical technique to measure the concentration of macular carotenoid pigments in the living human retina. Using a backscattering geometry and resonant molecular excitation in the visible, we measure the Raman peaks that originate from the single-and double-bond stretch vibrations of the pi -conjugated molecule's carbon backbone. The Raman signals scale linearly with carotenoid content, whereas the required laser excitation is well under safety limits for macular exposure. The Raman technique is objective and quantitative and may lead to a new method for rapid screening of carotenoid pigment levels in large human populations that are at risk for vision loss from age-related macular degeneration, the leading cause of blindness of the elderly in the United States. (C) 2001 Optical Society of America OCIS coder;: 170.3890, 170.4470, 160.4890, 290.5860, 300.6450, 330.4300.