In silico analyses suggest the cardiac ganglion of the lobster, Homarus americanus, contains a diverse array of putative innexin/innexin-like proteins, including both known and novel members of this protein family
In silico analyses suggest the cardiac ganglion of the lobster, Homarus americanus, contains a diverse array of putative innexin/innexin-like proteins, including both known and novel members of this protein family
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DOI：
10.1007/s10158-020-0238-6
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发表时间：
2020-03-02
影响因子：
--
通讯作者：
Dickinson, Patsy S.
中科院分区：
文献类型：
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作者：
Christie, Andrew E.;Hull, J. Joe;Dickinson, Patsy S.
缝隙连接是连接相邻细胞的物理通道，允许小分子/离子在耦合单元的细胞质之间流动。Innexin/Innexin样蛋白负责无脊椎动物缝隙连接的形成。在神经系统内，缝隙连接通常起到电突触的作用，为电耦合神经元之间的活动提供了一种协调手段。虽然一些缝隙连接允许小分子/离子在耦合的细胞之间双向流动，但另一些缝隙连接只允许单向流动或优先允许流动。缝隙连接中存在的内联蛋白的补体决定了它的特定性质。因此，了解内联蛋白多样性是了解物种/系统中电性耦合的全部潜力的关键。十足类甲壳动物心脏神经节(CG)控制心肌收缩，是一个简单的模式生成神经网络，其电路元件之间存在广泛的电耦合。在龙虾Homarus americanus中，先前的工作表明成年神经元innexin补体由六个innexin组成(Homam-Inx1-4和Homam-Inx6-7)。在这里，使用美洲钩吻线虫CG特异性转录组，我们探索了龙虾神经系统这一部分的innexin补体。除Homam-Inx4外，所有先前描述的内联蛋白似乎都在美洲钩端螺旋体中表达。此外，还鉴定了编码7个新的内联蛋白(Homam-Inx8-14)的转录本，其中4个(Homam-Inx8-11)具有多种剪接变体，例如6个Homam-Inx8。综上所述，这些数据表明，龙虾神经系统的innexin补体，特别是CG，可能比以前报道的要大得多，这表明美洲对虾有可能扩大缝隙连接多样性和功能。
Gap junctions are physical channels that connect adjacent cells, permitting the flow of small molecules/ions between the cytoplasms of the coupled units. Innexin/innexin-like proteins are responsible for the formation of invertebrate gap junctions. Within the nervous system, gap junctions often function as electrical synapses, providing a means for coordinating activity among electrically coupled neurons. While some gap junctions allow the bidirectional flow of small molecules/ions between coupled cells, others permit flow in one direction only or preferentially. The complement of innexins present in a gap junction determines its specific properties. Thus, understanding innexin diversity is key for understanding the full potential of electrical coupling in a species/system. The decapod crustacean cardiac ganglion (CG), which controls cardiac muscle contractions, is a simple pattern-generating neural network with extensive electrical coupling among its circuit elements. In the lobster, Homarus americanus, prior work suggested that the adult neuronal innexin complement consists of six innexins (Homam-Inx1-4 and Homam-Inx6-7). Here, using a H. americanus CG-specific transcriptome, we explored innexin complement in this portion of the lobster nervous system. With the exception of Homam-Inx4, all of the previously described innexins appear to be expressed in the H. americanus CG. In addition, transcripts encoding seven novel putative innexins (Homam-Inx8-14) were identified, four (Homam-Inx8-11) having multiple splice variants, e.g., six for Homam-Inx8. Collectively, these data indicate that the innexin complement of the lobster nervous system in general, and the CG specifically, is likely significantly greater than previously reported, suggesting the possibility of expanded gap junction diversity and function in H. americanus.