Specificity and cross-reactivity of monoclonal antibodies reactive with the core and lipid A regions of bacterial lipopolysaccharide.
Specificity and cross-reactivity of monoclonal antibodies reactive with the core and lipid A regions of bacterial lipopolysaccharide.
复制标题
与细菌脂多糖的核心和脂质 A 区域反应的单克隆抗体的特异性和交叉反应性。
DOI：
10.1093/infdis/159.2.168
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Guelde,G
中科院分区：
文献类型：
--
作者：
Pollack,M;Chia,JK;Koles,NL;Miller,M;Guelde,G
制备了29种针对大肠杆菌和明尼苏达沙门氏菌核心区和脂质A区抗原决定因子的小鼠单克隆抗体（mab）。至少鉴定出8种不同的MAb特异性。具有这些特异性的单克隆抗体识别的表位定位在核心低聚糖的己糖、己糖和2-酮-3-脱氧-d-甘露糖-辛糖酸区域和脂质a上。两组单克隆抗体对类似但不同的核心和脂质a相关表位表现出多特异性。一些核心反应性单克隆抗体与相应的抗体发生交叉反应。大肠杆菌和沙门氏菌通过突变化学型；其他则是特定的forE。coliJ5有限合伙人。脂质A特异性单克隆抗体与来自不同来源的游离脂质A反应。少数单克隆抗体与光滑的LPS反应。抗体的交叉反应性受到种间和种内共价核心结构差异以及o侧链和核心糖覆盖的表位隐藏的限制。核心脂质A复合物特异性单克隆抗体的交叉反应性和抗内毒素特性可能受到这种单克隆抗体无法识别“天然”LPS上的决定因子的限制。
Twenty-nine murine monoclonal antibodies (MAbs) were prepared against antigenic determinants in the core and lipid A regions ofEscherichia coliandSalmonella minnesotalipopolysaccharide (LPS). At least eight distinct MAb specificities were identified. Epitopes recognized by MAbs bearing these specificities were localized in the hexose, heptose, and 2-keto-3-deoxy-d-manno-octulosonic acid regions of the core oligosaccharide and on lipid A. Two groups of MAbs exhibited multispecificity for similar but distinct core- and lipid A-related epitopes. Some core-reactive MAbs cross-reacted with correspondingE. coliandSalmonellarough mutant chemotypes; others were specific forE. coliJ5 LPS. Lipid A-specific MAbs reacted with free lipid A from diverse sources. Few MAbs reacted with smooth LPS. Antibody cross-reactivity was restricted by inter- and intraspecies differences in covalent core structure and by epitope concealment by overlying O-side chain and core sugars. The putative cross-reactive and antiendotoxic properties of MAbs specific for the core-lipid A complex may be limited by the inability of such MAbs to recognize determinants on “native” LPS.