Differential distributions of neuropeptides in hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla in the rat
Differential distributions of neuropeptides in hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla in the rat
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DOI：
10.1016/j.neulet.2013.09.070
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发表时间：
2013-11-27
影响因子：
2.5
通讯作者：
Lee, So Yeong
中科院分区：
文献类型：
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作者：
Lee, Seul Ki;Ryu, Pan Dong;Lee, So Yeong
下丘脑室旁核（PVN）在内分泌和自主神经系统中发挥着重要的调节作用。它具有解剖学和功能上不同的细胞群，其中一个投射到延髓头端腹外侧（PVN-RVLM），并参与心血管系统的交感神经调节。采用单细胞RT-PCR结合逆行追踪技术，研究大鼠PVN-RVLM神经元内肽的表达和分布规律。大约80%的PVN-RVLM神经元表达催产素（OT），60%表达强啡肽（Dyn）。PVN-RVLM神经元各亚群之间肽的分布存在差异。后小细胞亚组主要表达OT;背帽亚组与其他亚组相比含有更少的加压素（VP）。此外，PVN-RVLM神经元优势共表达多种肽。这些结果表明，肽可能有助于复杂的反应PVN的各种刺激。(C)2013爱思唯尔爱尔兰有限公司版权所有。
The hypothalamic paraventricular nucleus (PVN) exerts a major regulatory role in the endocrine and the autonomic system. It has anatomically and functionally distinct cell populations, one of which projects to the rostral ventrolateral medulla (PVN-RVLM) and are involved in the sympathetic regulation of the cardiovascular system. To investigate the expression and the distributional patterns of peptides in the PVN-RVLM neurons of rats, we used single cell RT-PCR analysis in combination with retrograde tracing. Approximately 80% of PVN-RVLM neurons expressed oxytocin (OT) and 60% expressed dynorphin (Dyn). There were differential distributions of peptides between the subdivisions of PVN-RVLM neurons. The posterior parvocellular subdivision predominantly expressed OT; the dorsal cap subdivision contained much less vasopressin (VP) compared to other subdivisions. In addition, PVN-RVLM neurons preponderantly co-expressed multiple peptides. These results suggest that peptides may contribute to elaborate responses of PVN to various stimuli. (C) 2013 Elsevier Ireland Ltd. All rights reserved.