Identification of a novel Rhizopus-specific antigen by screening with a signal sequence trap and evaluation as a possible diagnostic marker of mucormycosis
Identification of a novel Rhizopus-specific antigen by screening with a signal sequence trap and evaluation as a possible diagnostic marker of mucormycosis
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通过信号序列陷阱筛选鉴定新型根霉特异性抗原并评估其作为毛霉菌病的可能诊断标志物
DOI：
10.1093/mmy/myw146
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发表时间：
2017
期刊：
影响因子：
2.9
通讯作者：
Kaneko Y. and Kakeya H.
中科院分区：
文献类型：
--
作者：
Sato K.;Oinuma K.;Niki M.;Yamagoe S.;Miyazaki Y.;Asai K.;Yamada K.;Hirata K.;Kaneko Y. and Kakeya H.
毛霉菌病是第二种最常见的霉菌感染，通常与其他侵袭性霉菌感染（如曲霉病）难以区分。虽然适当的抗真菌治疗在感染的早期阶段是有效的，但没有可靠的诊断方法来做出决策。因此，有必要开发一种高效的方法，可以快速，准确地检测毛霉菌病。本研究利用逆转录病毒介导表达的信号序列捕获法（SST-REX），对毛霉病最常见的病原菌--毛霉根霉（Rhizopus sp.）的分泌蛋白或膜结合蛋白进行了研究。在鉴定的蛋白质中，选择Rhizopus特异性抗原作为候选物，并使用R.感染水稻的老鼠在从SST-REX文库获得的302个克隆中，选择一种假设蛋白（23 kDa，命名为“蛋白RSA”）作为候选物，因为其克隆的最高流行率。与未感染小鼠相比，在感染小鼠的血清和肺匀浆中检测到蛋白RSA的浓度显著更高。我们的研究表明，RSA蛋白可能是一个有前途的生物标志物。水稻感染SST-REX可用于难治性霉菌感染的潜在真核生物标志物的全面筛选。
Mucormycosis is the second most common mould infection, often indistinguishable from other invasive mould infections such as aspergillosis. Although an appropriate antifungal therapy is effective at an early stage of the infection, there is no reliable diagnostic method for decision making. Thus, it is necessary to develop an efficient method that can detect mucormycosis rapidly and accurately. We searched for secreted or membrane-bound proteins ofRhizopus oryzae, which is the most common pathogen of mucormycosis, using the method of a signal sequence trap by retrovirus-mediated expression (SST-REX). Among the identified proteins, aRhizopus-specific antigen was selected as a candidate, and efficacy of this specific antigen was evaluated usingR. oryzae–infected mice. Of 302 clones obtained from the SST-REX library, a hypothetical protein (23 kDa, named “protein RSA”) was selected as a candidate because of its highest prevalence of clones. Protein RSA was detected at significantly higher concentrations in serum and in lung homogenates of the infected mice as compared to those of uninfected mice. Our study indicates that protein RSA may be a promising biomarker ofR. oryzaeinfection. SST-REX may be useful for comprehensive screening of prospective eukaryotic biomarkers of intractable mould infections.