Use of Rhizobium leguminosarum as a potential biofertilizer for Lactuca sativa and Daucus carota crops
Use of Rhizobium leguminosarum as a potential biofertilizer for Lactuca sativa and Daucus carota crops
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DOI：
10.1002/jpln.201300116
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发表时间：
2013-12-01
影响因子：
2.5
通讯作者：
Rivas, Raul
中科院分区：
文献类型：
--
作者：
Flores-Felix, Jose D.;Menendez, Esther;Rivas, Raul
微生物生物肥料通过减少化学肥料的使用，正在成为可持续农业的有效工具。然而，每种特定植物-微生物相互作用的知识对于正确应用至关重要。本研究以一株分离自菜豆根瘤的根瘤菌PEPV 16为材料，分析其促生机理。该菌株能够产生铁载体和吲哚乙酸，并溶解磷酸盐。共聚焦显微镜观察表明，该菌株能够在两种园艺作物莴苣（Lactucasativa L.）（莴苣）和胡萝卜（Daucus carota L.）（胡萝卜）。菌株PEPV 16还能够促进两种植物物种的植物生长，分别增加莴苣和胡萝卜的芽和根的干物质，以及增加两种植物物种的可食用部分中的N和P的吸收。这些数据证实了根瘤菌作为非豆科植物生物肥料的适用性。
Microbial biofertilizers are becoming an effective tool for sustainable agriculture by means of the reduction of the use of chemical fertilizers. However, the knowledge of each specific plant-microorganism interaction is essential for a correct application. In this study, we analyzed the in vitro plant-growth-promotion mechanisms of a Rhizobium leguminosarum strain named PEPV16 isolated from Phaseolus vulgaris nodules. This strain was able to produce siderophores and indole acetic acid and to solubilize phosphate. Confocal microscopy showed that this strain was able to colonize the roots of two horticultural crops, Lactuca sativa L. (lettuce) and Daucus carota L. (carrot). Strain PEPV16 was also able to promote the plant growth of both plant species increasing the dry matter of shoots and roots of lettuce and carrots, respectively, as well as to increase the uptake of N and P in the edible parts of both plant species. These data confirmed the suitability of Rhizobium as biofertilizer for nonlegumes.