Mouse oocytes differentiate through organelle enrichment from sister cyst germ cells
Mouse oocytes differentiate through organelle enrichment from sister cyst germ cells
复制标题
DOI：
10.1126/science.aad2156
复制
发表时间：
2016-04-01
期刊：
影响因子：
56.9
通讯作者：
Spradling, Allan C.
中科院分区：
文献类型：
--
作者：
Lei, Lei;Spradling, Allan C.
卵母细胞在不同物种中通过接受来自姐妹生殖细胞的细胞器和细胞质而分化，同时加入生殖细胞包囊或合胞体。小鼠原始生殖细胞形成生殖细胞包囊，但包囊在卵子发生中的作用尚不清楚。我们发现，小鼠生殖细胞从邻近的包囊细胞获得细胞器，并建立一个Balbiani体成为卵母细胞，而nurselike生殖细胞死亡。细胞器的运动，Balbiani体的形成，卵母细胞的命运决定选择性地阻止低水平的微管依赖性运输抑制剂。胞囊内的膜破裂和胞囊破裂样过程与细胞器转移到卵母细胞中有关，这些事件让人想起果蝇中的护士细胞倾倒。我们建议，细胞质和细胞器运输在哺乳动物卵母细胞分化中起着进化上保守和功能上重要的作用。
Oocytes differentiate in diverse species by receiving organelles and cytoplasm from sister germ cells while joined in germline cysts or syncytia. Mouse primordial germ cells form germline cysts, but the role of cysts in oogenesis is unknown. We find that mouse germ cells receive organelles from neighboring cyst cells and build a Balbiani body to become oocytes, whereas nurselike germ cells die. Organelle movement, Balbiani body formation, and oocyte fate determination are selectively blocked by low levels of microtubule-dependent transport inhibitors. Membrane breakdown within the cyst and an apoptosis-like process are associated with organelle transfer into the oocyte, events reminiscent of nurse cell dumping in Drosophila. We propose that cytoplasmic and organelle transport plays an evolutionarily conserved and functionally important role in mammalian oocyte differentiation.