SORBITOL AND SUCROSE AS CARBON SOURCE FOR CALLUS-CULTURE OF SOME SPECIES OF ROSACEAE
SORBITOL AND SUCROSE AS CARBON SOURCE FOR CALLUS-CULTURE OF SOME SPECIES OF ROSACEAE
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DOI：
10.1139/b76-054
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
CHONG, C
中科院分区：
文献类型：
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作者：
COFFIN, R;TAPER, CD;CHONG, C
以蔷薇科17种植物的茎段愈伤组织为材料，在含3%蔗糖和（或）3%山梨醇的培养基上进行了诱导试验。叶蜂属1种;苹果属1种;李属9种;梨属Pyrus，1 sp.;花楸属2种;在海棠（Malus pumilavar.niedzwetzkyana）的情况下，蔗糖和山梨醇培养基对愈伤组织的诱导同样有效，并且在这些培养基上保持相等的生长。香绣线菊的愈伤组织仅在蔗糖培养基上发生，而扁桃的愈伤组织在两种培养基上均未形成。与所有其他物种，愈伤组织的启动和（或）给了更好的生长进一步继代蔗糖比山梨醇培养基上，除了forPrunus persica（桃），它生长得更好的山梨醇。
Initiation of stem callus cultures on a nutrient medium with either 3% sucrose and (or) 3% sorbitol as carbon source was attempted with 17 species selected from the following genera of the Rosaceae:Amelanchier, two spp.;Crataegus, one sp.;Malus, one sp.;Prunus, nine spp.;Pyrus, one sp.;Sorbus, two spp.; andSpiraea, one sp. In the case ofMalus pumilavar.niedzwetzkyana(crabapple), sucrose and sorbitol media were equally effective in callus initiation, and equal growth was maintained on these media. Callus ofSpiraea vanhoutteiwas initiated only on sucrose medium and no callus ofPrunus tenellaformed on either medium. With all other species, callus was initiated and (or) gave better growth on further subculture on sucrose than on sorbitol medium, except forPrunus persica(peach), which grew better on sorbitol.