Cartilage mechanics in the guinea pig model of osteoarthritis studied with an osmotic loading method.
Cartilage mechanics in the guinea pig model of osteoarthritis studied with an osmotic loading method.
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DOI：
10.1016/j.joca.2004.01.007
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发表时间：
2004-05
影响因子：
7
通讯作者：
C. Flahiff;V. Kraus;J. Huebner;L. Setton
中科院分区：
文献类型：
--
作者：
C. Flahiff;V. Kraus;J. Huebner;L. Setton
目的探讨Hartley豚鼠自发性骨关节炎（osteoarthritis，OA）模型中关节软骨的材料特性。方法对12个月龄豚鼠膝关节内侧股骨髁和胫骨平台的软骨-骨标本进行渗透负荷。位点匹配的溶胀应变和固定电荷密度值用于软骨溶胀的三相理论模型中，以确定软骨固体基质的模量。软骨退化的程度进行了评估，在相邻的组织切片使用半定量的组织学分级scheme.ResultsDecreased值的模量和表面区固定电荷密度与软骨退化的等级增加。模量的下降反映了胶原蛋白基质的损伤，从而引起更大的肿胀strain.ConclusionHistological证据的软骨变性与受损的软骨力学在老化哈特利豚鼠。
ObjectiveTo determine the material properties of articular cartilage in the Hartley guinea pig model of spontaneous osteoarthritis.MethodsCartilage-bone samples from the medial femoral condyle and tibial plateau of 12 month-old guinea pig knees were subjected to osmotic loading. Site-matched swelling strains and fixed charge density values were used in a triphasic theoretical model for cartilage swelling to determine the modulus of the cartilage solid matrix. The degree of cartilage degeneration was assessed in adjacent tissue sections using a semi-quantitative histological grading scheme.ResultsDecreased values for both moduli and surface zone fixed charge density were associated with increasing grades of cartilage degeneration. Decreases in moduli reflect damage to the collagen matrix, which give rise to greater swelling strains.ConclusionHistological evidence of cartilage degeneration was associated with impaired cartilage mechanics in the aging Hartley guinea pig.