Hippocampal neurogenesis is reduced by sleep fragmentation in the adult rat
Hippocampal neurogenesis is reduced by sleep fragmentation in the adult rat
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DOI：
10.1016/j.neuroscience.2007.05.030
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发表时间：
2007-08-10
期刊：
影响因子：
3.3
通讯作者：
McGinty, D.
中科院分区：
文献类型：
--
作者：
Guzman-Marin, R.;Bashir, T.;McGinty, D.
成年海马齿状回（DG）是持续神经发生的部位。这个过程受到各种生理和经验刺激的影响，包括完全睡眠剥夺（TSD）。在人类中，睡眠碎片（SF）是比TSD更常见的睡眠状况。SF与几种流行疾病有关。我们评估了一个假设，SF会抑制成年大鼠DG的成年神经发生。使用间歇性跑步机系统;跑步机开启3 s，关闭30 s（SF）。对于睡眠片段控制（SFC），跑步机上15分钟和关闭150分钟。SF进行了三个持续时间：1，4和7天。为了标记增殖细胞，在每个实验结束前2小时注射胸苷类似物5-溴-2-脱氧尿苷（BrdU）。还研究了内在增殖标记物Ki 67的表达。SF大鼠表现出非快速眼动（NREM）睡眠发作次数的增加，而在此阶段花费的时间百分比没有变化。BrdU阳性细胞和Ki 67阳性细胞减少约70%（P
The adult hippocampal dentate gyrus (DG) is a site of continuing neurogenesis. This process is influenced by a variety of physiological and experiential stimuli including total sleep deprivation (TSD). In humans, sleep fragmentation (SF) is a more common sleep condition than TSD. SF is associated with several prevalent diseases. We assessed a hypothesis that SF would suppress adult neurogenesis in the DG of the adult rat. An intermittent treadmill system was used; the treadmill was on for 3 s and off for 30 s (SF). For sleep fragmentation control (SFC), the treadmill was on for 15 min and off for 150 min. SF was conducted for three durations: 1, 4 and 7 days. To label proliferating cells, the thymidine analog, 5-bromo-2-deoxyuridine (BrdU), was injected 2 h prior to the end of each experiment. Expression of the intrinsic proliferative marker, Ki67, was also studied. SF rats exhibited an increased number of non-rapid eye movement (NREM) sleep bouts with no change in the percent of time spent in this stage. The numbers of both BrdU-positive cells and Ki67-positive cells were reduced by similar to 70% (P