A new individualization marker of sweat: deoxyribonuclease I (DNase I) polymorphism.

A new individualization marker of sweat: deoxyribonuclease I (DNase I) polymorphism.
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一种新的汗液个体化标记:脱氧核糖核酸酶 I (DNase I) 多态性。

DOI:
10.1520/jfs14012j
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发表时间:
1996
影响因子:
1.6
通讯作者:
K. Kishi
K. Kishi
中科院分区:
医学4区
文献类型:
--
作者:
T. Yasuda;H. Takeshita;K. Sawazaki;D. Nadano;R. Iida;S. Miyahara;K. Kishi

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我们第一次证实,生物化学和免疫学,脱氧核糖核酸酶I(DNase I)在人类液体汗液中的存在。将汗液样品在聚丙烯酰胺凝胶(pH 3.5至5)上等电聚焦,然后进行干燥琼脂糖膜覆盖检测,用于确定汗液DNA酶I的表型。由于这种检测方法不仅具有高灵敏度,而且具有高条带分辨率,因此可以从50至100 μ L的汗液样品中确定DNase I类型。唾液酸酶预处理汗液样品是分型的必要条件,可显著提高分型的灵敏度,同时简化同工酶谱。所有汗液样本中的DNA酶I类型与相应的血液、尿液和精液样本中发现的类型一致。因此,DNA酶I分型可以提供一种新的鉴别特征,在法医检验的汗水。
We confirmed for the first time, both biochemically and immunologically, the existence of deoxyribonuclease I (DNase I) in human liquid sweat. Isoelectric focusing of sweat samples on polyacrylamide gels (pH 3.5 to 5), followed by dried agarose film overlay detection, was used to determine the phenotypes of sweat DNase I. Because this detection method not only had high sensitivity, but also high band resolution, it was possible to determine DNase I types from sweat samples of 50 to 100 microL. Pretreatment of sweat samples with sialidase was essential for typing to enhance markedly the sensitivity accompanied by simplification of the isozyme pattern. The DNase I types in all sweat samples were consistently related to the types found in corresponding blood, urine, and semen samples. DNase I typing could, therefore, provide a novel discriminant characteristic in the forensic examination of sweat.
DNA 降解酶 I (DNase I)
DOI: --
发表时间: 2004
期刊: 日本臨床 62・増11
影响因子: --
作者:
岸 紘一郎
通讯作者: 岸 紘一郎
DOI: --
发表时间: --
期刊:
影响因子: --
作者:
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