Genistein Inhibits Colonic Goblet Cell Loss and Colorectal Inflammation Induced by Salmonella Typhimurium Infection
Genistein Inhibits Colonic Goblet Cell Loss and Colorectal Inflammation Induced by Salmonella Typhimurium Infection
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DOI：
10.1002/mnfr.202100209
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发表时间：
2021-06-29
影响因子：
5.2
通讯作者：
Zhang, Bingkun
中科院分区：
文献类型：
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作者：
He, Yang;Ayansola, Hammed;Zhang, Bingkun
范围沙门氏菌是主要的食源性致病菌，可感染肠道上皮细胞，引起结肠炎。染料木黄酮具有多种生物学活性，可通过多种途径减轻葡聚糖硫酸钠诱导的结肠炎，但其对致病菌引起的结肠炎的保护作用尚不清楚。方法和结果本研究探讨了染料木黄酮对沙门氏菌感染引起的结肠炎的保护作用。沙门氏菌通过激活环氧合酶-2/前列腺素E2引起结肠炎症，染料木黄酮抑制鼠伤寒沙门氏菌感染引起的结肠炎。沙门氏菌感染增加结肠粘膜损伤、增殖细胞和杯状细胞损失，而染料木黄酮的施用解决了这些病理变化。此外，进一步证明沙门氏菌引起与杯状细胞丢失相关的严重结肠炎，并激活宿主隐窝干细胞修复受损的上皮。沙门氏菌感染抑制宿主哺乳动物雷帕霉素靶标，激活轻链3 II途径诱导自噬以消除病原菌。染料木黄酮增加粪便中的乳酸杆菌，减少沙门氏菌的定植，从而抑制沙门氏菌感染引起的结肠炎。结论金雀异黄素通过调节肠道菌群和肠道干细胞发育，减轻沙门氏菌感染引起的结肠炎，抑制杯状细胞丢失。
Scope Salmonella is the main food-borne pathogen, which can infect intestinal epithelial cells and causes colitis. Genistein has a variety of biological activities that alleviates colitis induced by sodium dextran sulfate in a variety of ways, but its protective effects on colitis caused by pathogenic bacteria are still unknown. Methods and Results This study explores the protective effect of genistein in reducing colitis caused by Salmonella infection. Salmonella causes colon inflammation through activating cyclooxygenase-2/prostaglandin E2, and genistein inhibits colitis caused by Salmonella typhimurium infection. Salmonella infection increases colonic mucosal damage, proliferating cells, and goblet cell loss, while the administration of genistein solves these pathological changes. In addition, it is further proved that Salmonella causes severe colitis related to goblet cell loss and activates the host crypt stem cells to repair the damaged epithelium. Salmonella infection inhibites the host mammalian target of rapamycin, activates light chain 3 II pathways to induce autophagy to eliminate pathogenic bacteria. Genistein increases Lactobacillus in feces and reduces Salmonella colonization to inhibit colitis induces by Salmonella infection. Conclusion This study demonstrates genistein alleviated colitis and inhibites the goblet cell loss causes by Salmonella infection through regulating the gut bacteria and intestinal stem cell development.