Subclasses of human IgG anti-Fab antibodies: parameters for optimum detection.
Subclasses of human IgG anti-Fab antibodies: parameters for optimum detection.
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人 IgG 抗 Fab 抗体的亚类：最佳检测参数。
DOI：
10.1159/000233915
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Stevens,RH
中科院分区：
文献类型：
--
作者：
Persselin,JE;Keld,B;Fried,L;Stevens,RH
在这项研究中，我们已经定义了抗Fab抗体在类风湿关节炎患者的血清中的最佳检测所需的参数。我们发现大多数抗Fab抗体是IgG 3和IgG 4亚类，使用多克隆异源抗人IgG抗血清不能最佳地检测到这些亚类;相反，需要亚类特异性抗体。此外，我们确定，通过针对尿素透析3-7天来解离循环免疫复合物也是检测这些抗体所需的。最后，我们已经表明，解离的复合物可以与它们的靶Fab分子重组，因此通过色谱聚焦将抗Fab抗体与其他免疫球蛋白分离可以增强这些抗体的检测。当满足上述条件时，确定可以在类风湿性关节炎和正常血清中检测到IgG抗Fab抗体，并且酸性IgG 3和IgG 4亚类占优势。然而，与正常血清相比，类风湿性关节炎血清中的IgG 3水平高10.5倍（p <0.05），IgG 4水平高5倍（p <0.01）。
In this study we have defined the parameters needed for the optimum detection of anti-Fab antibodies in the serum of patients with rheumatoid arthritis. We have found that the majority of the anti-Fab antibodies are of the IgG3 and IgG4 subclasses which were not optimally detected using polyclonal heterologous anti-human IgG antisera; subclass-specific antibodies instead were needed. Additionally we determined that dissociation of circulating immune complexes by dialysis against urea for 3–7 days was also needed for the detection of these antibodies. Lastly we have shown that the dissociated complexes can recombine with their target Fab molecules, and therefore separation of the anti-Fab antibodies from the other immunoglobulins by chromatofocusing may enhance the detection of these antibodies. When the above conditions were fulfilled it was determined that IgG anti-Fab antibodies could be detected in rheumatoid arthritis and normal sera and that acidic IgG3 and IgG4 subclasses predominated. However, IgG3 levels were 10.5-fold higher in rheumatoid arthritis sera (p <0.05) and IgG4 levels 5-fold higher (p <0.01) than in normal sera.