In vivo fluence rate effect in photodynamic therapy of early cancers with tetra(m-hydroxyphenyl)chlorin
In vivo fluence rate effect in photodynamic therapy of early cancers with tetra(m-hydroxyphenyl)chlorin
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DOI：
10.1111/j.1751-1097.1996.tb01862.x
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发表时间：
1996-12-01
影响因子：
3.3
通讯作者：
Monnier, P
中科院分区：
文献类型：
--
作者：
Blant, SA;Woodtli, A;Monnier, P
光动力学治疗中有几个参数影响临床试验并影响治疗结果，除了药物剂量、光剂量、药-光间隔等变量外，注量率是影响治疗结果的参数。在本研究中，我们以7，12-二甲基苯并(A)菲诱导的叙利亚仓鼠颊囊早期鳞状细胞癌为肿瘤模型，评价了第二代光敏剂四(间羟基苯基)氯(MTHPC)的注入率效应。在注射0.5 mg/kg mTHPC后，分别在4天和8天两个药物光间隔进行照射试验，波长和光剂量与临床试验中常规应用的波长和光剂量相适应。在652 nm处进行照射，剂量范围从8到20 J/cm(2)，剂量率为15和150 mW/cm(2)，也进行类似的测试，514 nm，剂量率为80 J/cm(2)，剂量率从25到125 mW/cm(2)，在波长和药物光间隔下，对于给定的剂量，较高的剂量率导致肿瘤和健康黏膜的组织损伤较小，但是，较低的剂量率产生的治疗选择性略低，本研究证实了光通量率在临床PDT中具有重要意义。
Several parameters affect clinical trials in photodynamic therapy and influence the therapeutic outcome, Beside drug dose, light dose, drug-light interval and other variables, the fluence rate is a parameter that can influence the therapeutic results, In this study we have evaluated the fluence rate effect with a second-generation photosensitizer, tetra(m-hydroxyphenyl)chlorin (mTHPC) using a 7,12-dimethylbenz(a)anthracene induced early squamous cell carcinoma of the Syrian hamster cheek pouch as a tumor model. Following injection of 0.5 mg/kg of mTHPC, irradiation tests were performed at two drug-light intervals, 4 and 8 days, Wavelength and light dose were adapted from those applied routinely in clinical trials. Irradiations at 652 nm were carried out with fluences ranging from 8 to 20 J/cm(2) delivered at fluence rates of 15 and 150 mW/cm(2), Similar tests were also performed at 514 nm with a fluence of 80 J/cm(2) delivered at fluence rates ranging from 25 to 125 mW/cm(2), At both wavelengths and drug-light intervals for a given fluence, the higher fluence rates resulted in less tissue damage in tumor and healthy mucosae, However, the lower fluence rates yielded slightly less therapeutic selectivity, This study confirms that the fluence rate is of major importance in clinical PDT.