Separation and identification of the organic acids in Angelicae Radix and Ligustici Rhizoma by HPLC and CE
Separation and identification of the organic acids in Angelicae Radix and Ligustici Rhizoma by HPLC and CE
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DOI：
10.1002/jssc.200600136
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发表时间：
2006-11-01
影响因子：
3.1
通讯作者：
Sheu, Shuenn-Jyi
中科院分区：
文献类型：
--
作者：
Huang, Wen-Ying;Sheu, Shuenn-Jyi
伞形科植物中的两种重要的活性成分--黄芪（AR）和甘草（LR）均含有相似的有机酸。其中烟酸、原儿茶酸、邻苯二甲酸、亚叶酸、对羟基苯甲酸、叶酸、香草酸、咖啡酸和阿魏酸等9种有机酸被高效液相色谱和毛细管电泳分离。检测到210边缘。在HPLC中用含有20 mMKH 2 PO 4（pH3.5）和H2O-CH 3CN的线性梯度洗脱和在CE中用含有10 mMLTAC、2 mMNa 2 HPO 4、9mMNa 2B 4 O 7（pH9.56）和CH 3CN的缓冲溶液洗脱被发现是用于该分离的最有效的洗脱剂。用液相色谱法和毛细管电泳法分别在60 min和20 min内测定了AR和LR粗提物中9种成分的含量。通过LC-MS对色谱图中各峰的结构进行了鉴定。考察了缓冲液对两种方法分离和验证的影响。
Angelicae Radix (AR) and Ligustici Rhizoma (LR) are both derived from the Umbelliferae plants and contain similar organic acids as their bioactive compounds. Nine of these organic acids, including nicotinic acid, protocatechuic acid, phthalic acid, folinic acid, p-hydroxybenzoic acid, folic acid, vanillic acid, caffeic acid, and ferulic acid were separated by HPLC and CE. Detection at 210 rim. with a linear gradient containing 20 mM KH2PO4 (pH 3.5) and H2O - CH3CN in HPLC and with a buffer solution containing 10 mM LTAC, 2 mM Na2HPO4, 9 mM Na2B4O7 (pH 9.56), and CH3CN in CE were found to be the most efficient eluents for this separation. The contents of the nine components in crude extracts of either AR or LR could easily be determined within 60 min by LC and within 20 min by CE. The structures of the individual peaks in the LC chromatogram were identified by LC-MS. The effects of buffers on the separation and validation of the two methods were examined.