DIFFERENTIAL EFFECT OF DIETARY-FAT SATURATION AND CHOLESTEROL ON HEPATIC APOLIPOPROTEIN GENE-EXPRESSION IN RATS
DIFFERENTIAL EFFECT OF DIETARY-FAT SATURATION AND CHOLESTEROL ON HEPATIC APOLIPOPROTEIN GENE-EXPRESSION IN RATS
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DOI：
10.1016/0021-9150(94)90039-6
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发表时间：
1994-07-01
期刊：
影响因子：
5.3
通讯作者：
PALACIOSALAIZ, E
中科院分区：
文献类型：
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作者：
OSADA, J;FERNANDEZSANCHEZ, A;PALACIOSALAIZ, E
在雄性大鼠中研究了膳食胆固醇和脂肪饱和度对肝载脂蛋白 A-I、A-II、A-IV、B、C-I、C-III、E 和 LDL 受体 mRNA 水平的影响。动物维持 2 个月的高脂肪饮食（40%，w/w），其中含有 0.1% 胆固醇。两组对照动物接受食物饮食或添加 0.1% 胆固醇的食物，而实验组则接受椰子油、玉米油或橄榄油作为脂肪补充剂。饲喂橄榄油的动物的肝载脂蛋白 A-I 水平高于对照胆固醇组（1.6 +/- 0.3 与 0.8 +/- 0.2）。食用饱和（椰子）和不饱和（玉米和橄榄）脂肪饮食的动物的 Apo E mRNA 水平比对照胆固醇组高 50% 和 72%。 Apo B 和 apo C-I mRNA 水平不受实验条件影响。在存在胆固醇的组中，Apo A-IV mRNA 增加了 66% 至 127%。与对照组相比，玉米喂养组的 LDL 受体 mRNA 增加了 2 倍。这些结果表明，编码参与脂蛋白代谢的产物的基因的表达对膳食脂肪饱和度和胆固醇具有不同的敏感性。
The effects of dietary cholesterol and fat saturation on hepatic apolipoprotein A-I, A-II, A-IV, B, C-I, C-III, E and LDL receptor mRNA levels were studied in male rats. Animals were maintained for 2 months on a high fat diet (40%, w/w) containing 0.1% cholesterol. Two groups of control animals received either chow diet or chow plus 0.1%, cholesterol, while experimental groups received as their fat supplement coconut, corn or olive oil. Olive oil fed animals had higher levels of hepatic apo A-I than the control cholesterol group (1.6 +/- 0.3 vs. 0.8 +/- 0.2). Apo E mRNA levels were 50% and 72% higher in animals consuming the saturated (coconut) and unsaturated (corn and olive) fat diet than the control cholesterol group. Apo B and apo C-I mRNA levels were not affected by the experimental conditions. Apo A-IV mRNA increased between 66% and 127% in groups in which cholesterol was present. LDL receptor mRNA increased 2 times in the corn fed group compared with the control groups. These results indicate that the expression of genes coding for products involved in lipoprotein metabolism have a differential susceptibility to dietary fat saturation and cholesterol.