Cardiolipin on the surface of apoptotic cells as a possible trigger for antiphospholipid antibodies
Cardiolipin on the surface of apoptotic cells as a possible trigger for antiphospholipid antibodies
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DOI：
10.1046/j.1365-2249.2000.01353.x
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发表时间：
2000-11-01
影响因子：
4.6
通讯作者：
Valesini, G
中科院分区：
文献类型：
--
作者：
Sorice, M;Circella, A;Valesini, G
本研究提供的证据表明，心磷脂(CL)分子表达在凋亡细胞表面，并被抗磷脂抗体识别，抗磷脂抗体是从抗磷脂抗体综合征(APS)患者中提纯的。细胞膜纯化部分磷脂的高效薄层分析和CL特异性染料--壬基吖啶橙的阳性染色证实了CL在细胞表面的表达。这一发现与从APS患者中提纯的ACL IgG与凋亡细胞表面结合的观察结果相补充。染色显示成簇状分布，主要分布在表面小泡上。有趣的是，细胞荧光分析显示，细胞表面的CL暴露在DNA断裂之前。这些发现表明，细胞质膜上CL分子的暴露是细胞凋亡程序的早期事件，这可能是在体内触发了ACL的产生。
This study provides evidence that cardiolipin (CL) molecules are expressed on the surface of apoptotic cells and are recognized by antiphospholipid antibodies, purified from patients with the antiphospholipid antibody syndrome (APS). CL expression on cell surface was demonstrated by high performance thin layer chromatography analysis of phospholipids from plasma membrane purified fractions and by the positive staining with the CL-specific dye nonyl-acridine orange. This finding was complemented with the observation that aCL IgG purified from patients with APS bind to the surface of apoptotic cells. This staining shows a clustered distribution mostly localized on surface blebs. Interestingly, CL exposure on the cell surface preceded the DNA fragmentation, as shown by cytofluorimetric analysis. These findings demonstrate that exposure of CL molecules on the cell plasma membrane is an early event of the apoptotic cellular program that may represent an in vivo trigger for the generation of aCL.