Platelet-derived growth factor: identification of constituent polypeptide chains.
Platelet-derived growth factor: identification of constituent polypeptide chains.
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DOI：
10.1016/0006-291x(82)91941-6
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发表时间：
1982-01
影响因子：
3.1
通讯作者：
A. Johnsson;C. Heldin;B. Westermark;Å. Wasteson
中科院分区：
文献类型：
--
作者：
A. Johnsson;C. Heldin;B. Westermark;Å. Wasteson
疏水高效液相色谱法还原和烷基化125 I标记的血小板衍生生长因子（PDGF）导致两个不同的放射性成分的分离。其中一个命名为A，在十二烷基硫酸钠中通过聚丙烯酰胺凝胶电泳分离为四种，分子量分别为18，000，15，000，14，000和11，000。另一种组分，命名为B，只显示一种主要形式，Mr约为16，000。未标记的PDGF产生类似的产物模式。提出了一个分子模型，其中每个PDGF分子由一个A多肽和一个B多肽组成，通过二硫键连接。A链而不是B链具有可变的大小，导致完整PDGF分子中的不均匀性（Mr 28，000 - 33，000）。一些分子量异质性与完全生物活性相容。
Hydrophobic high-performance liquid chromatography of reduced and alkylated125I-labeled platelet-derived growth factor (PDGF) resulted in the separation of two distinct radioactive components. One of them, designated A, was resolved by polyacrylamide gel electrophoresis in sodium dodecyl sulfate, into four species with the molecular weights 18,000, 15,000, 14,000 and 11,000, respectively. The other component, designated B, showed only one major form with an Mrof about 16,000. Unlabeled PDGF yielded a similar pattern of products. A molecular model is proposed in which each molecule of PDGF consists of one A and one B polypeptide, linked by disulfide bonds. The A chain, but not the B chain, has a variable size, leading to inhomogeneity in the intact PDGF molecule (Mr28,000 – 33,000). Some molecular weight heterogeneity is compatible with full biological activity.