Characterization of DUF724 gene family in Arabidopsis thaliana
Characterization of DUF724 gene family in Arabidopsis thaliana
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拟南芥 DUF724 基因家族的特征
DOI：
10.1007/s11103-009-9551-5
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发表时间：
2010-01-01
影响因子：
5.1
通讯作者：
Ye, De
中科院分区：
文献类型：
--
作者：
Cao, Xi;Yang, Ke-Zhen;Ye, De
在植物分类群中发现了18个编码具有高度保守的未知功能结构域724（DUF 724）和未知结构域的蛋白质的基因，但在动物和真菌中没有发现。它们在许多不同的植物组织中活跃表达，暗示它们可能在植物中发挥重要作用。在这里，我们报告其结构组织，表达模式和蛋白质-蛋白质相互作用的表征。在拟南芥中，DUF 724基因在根、叶、茎尖分生组织、花粉囊和花粉粒中表达。拟南芥DUF 724的10个蛋白中至少有7个（AtDuf 1到AtDuf 10）定位于细胞核。其中三个（AtDuf 3，AtDuf 5和AtDuf 7）可能形成同源二聚体或均聚物，但不与同一家族的其他成员相互作用。结合DUF 724蛋白与FMRP在基本和特征分子结构上的显著相似性，推测DUF 724基因家族可能通过RNA转运参与植物细胞的极性生长。
Eighteen genes that encode the proteins with highly conserved Domain of Unknown Function 724 (DUF724) and Agenet domains were identified in plant taxa but not in animals and fungi. They are actively expressed in many different plant tissues, implying that they may play important roles in plants. Here we report the characterization of their structural organizations, expression patterns and protein-protein interactions. In Arabidopsis, the DUF724 genes were expressed in roots, leaves, shoot apical meristems, anthers and pollen grains. At least seven of the ten Arabidopsis DUF724 proteins (AtDuf1 to AtDuf10) were localized in nucleus. Three of them (AtDuf3, AtDuf5 and AtDuf7) may form homodimers or homopolymers, but did not interact with other members of the same family. Together with the significant similarity between DUF724 proteins and FMRP in the fundamental and characteristic molecular architecture, the results implies the DUF724 gene family may be involved in the polar growth of plant cells via transportation of RNAs.