THE COMPLETE SEQUENCE OF DROSOPHILA BETA-SPECTRIN REVEALS SUPRA-MOTIFS COMPRISING 8 106-RESIDUE SEGMENTS
THE COMPLETE SEQUENCE OF DROSOPHILA BETA-SPECTRIN REVEALS SUPRA-MOTIFS COMPRISING 8 106-RESIDUE SEGMENTS
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DOI：
10.1073/pnas.89.13.6187
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发表时间：
1992-07-01
影响因子：
11.1
通讯作者：
BRANTON, D
中科院分区：
文献类型：
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作者：
BYERS, TJ;BRANDIN, E;BRANTON, D
血影蛋白的α链和β链是同源的，但它们已经获得了不同的结构特征，这些结构特征协同作用以赋予多聚体其整体性质。每个血影蛋白亚基的一级氨基酸序列由串联重复的106个残基序支配。通过比较完整的果蝇-β-血影蛋白序列与其他血影蛋白，我们发现了证据表明，一个更高的顺序，848个氨基酸的超基序是串联重复的α和β-血影蛋白。这些数据表明，α-和β-血影蛋白，而不是独立地从编码祖先的106个残基的基序的序列进化而来，必须在建立一个由106个残基的基序中的8个组成的大的超基序后出现。我们的数据表明，产生α-和β-血影蛋白以及抗肌萎缩蛋白的祖先基因的片段结构。在α-血影蛋白和β-血影蛋白基因分裂后进化出的结构差异赋予了它们今天存在于其产物中的独立功能。
The alpha and beta-chains of spectrin are homologous, yet they have acquired different structural features that work in synergy to give the multimer its overall properties. The primary amino acid sequence of each spectrin subunit is dominated by tandemly repeated 106-residue motifs. By comparing the complete Drosophila-beta-spectrin sequence with other spectrins we have discovered evidence that a higher-order, 848-amino acid supra-motif is tandemly repeated in both alpha- and beta-spectrin. These data argue that alpha- and beta-spectrin, rather than evolving independently from sequences encoding the ancestral 106-residue motifs, must have arisen after the establishment of a large supra-motif composed of eight of the 106-residue motifs. Our data suggest the segment structure of a progenitor gene that gave rise to both alpha- and beta-spectrin as well as dystrophin. The structural differences that evolved after the split between the alpha- and beta-spectrin genes confer the independent functions that exist in their products to day.