Apparent difference in the roles of spleen and lymph nodes in the pathogenesis of host-versus-graft disease in murine parent/F1 chimeras.
Apparent difference in the roles of spleen and lymph nodes in the pathogenesis of host-versus-graft disease in murine parent/F1 chimeras.
复制标题
小鼠亲本/F1嵌合体中脾脏和淋巴结在宿主抗移植物疾病发病机制中的作用存在明显差异。
DOI：
10.1097/00007890-198709000-00022
复制
发表时间：
1987
期刊：
影响因子：
6.2
通讯作者：
Keller,LM
中科院分区：
文献类型：
--
作者：
HardJr,RC;Benson,LL;Keller,LM
宿主抗移植物（HVG）病是一种常见的致死性免疫缺陷综合征，可通过围产期接种半同种异体脾细胞在易感品系小鼠中诱导。为了确定供体细胞在RFM宿主脾脏和淋巴结中的分布和植入，对（TxRFM）F细胞的存在进行了连续试验，这些细胞通过其形成针对辣根过氧化物酶（HRP）的供体特异性抗体的能力和T染色体进行标记。用结合HIRP（HRPBC）的胞浆内抗体对细胞进行定量，用T标记物对中期分裂相进行定量，结果显示> 90%的供体细胞位于增生性淋巴结中。HRPBC数量的顺序变化模式与HRP抗体滴度的上升和下降相对应。供体细胞定位的显著差异表明宿主淋巴结和脾脏发挥不同的作用。淋巴结成为供体抗体的主要来源，
Host-versus-graft (HVG) disease is the often fatal immunodeficiency syndrome that can be induced in susceptible strains of mice by the perinatal inoculation of semiallogenic spleen cells. To determine the distribution and engraftment of the donor cells in the spleens and lymph nodes of RFM hosts, sequential tests were done for the presence of (TxRFM) F, cells marked by their ability to form donor-specific antibodies to horseradish peroxidase (HRP) and by the T chromosome. Quantitation of cells with intracytoplasmic antibody that bound HIRP (HRPBC) and of metaphases with the T marker showed that> 90% of donor cells were located in the hyperplastic lymph nodes. The pattern of sequential changes in numbers of HIRPBC corresponded with the rise and fall in titers of antibodies to HRP. The marked differences in localization of donor cells suggested that host nodes and spleens played different roles. The lymph nodes became the main sources of donor antibodies and the principal