Impact of the Heterogeneity in Free Chlorine, UV254, and Ozone Susceptibilities Among Coxsackievirus B5 on the Prediction of the Overall Inactivation Efficiency
Impact of the Heterogeneity in Free Chlorine, UV254, and Ozone Susceptibilities Among Coxsackievirus B5 on the Prediction of the Overall Inactivation Efficiency
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柯萨奇病毒 B5 中游离氯、UV254 和臭氧敏感性的异质性对总体灭活效率预测的影响
DOI：
10.1021/acs.est.0c07796
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发表时间：
2021
影响因子：
11.4
通讯作者：
Katayama Hiroyuki
中科院分区：
文献类型：
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作者：
Torii Shotaro;Miura Fuminari;Itamochi Masae;Haga Kei;Katayama Kazuhiko;Katayama Hiroyuki
病毒的消毒敏感性甚至在变异体之间也不同，但特定病毒基因型、种或属的灭活效率是根据其实验室菌株的敏感性确定的。目的是评价柯萨奇病毒B5（CVB 5）的13种变异体对游离氯、UV 254和臭氧的亲和性的变异性，并建立模型预测异质性CVB 5的整体灭活。我们的研究结果表明，在游离氯，UV 254，和臭氧，分别高达3.4倍，1.3倍，和1.8倍的结晶度不同。基因组B中的CVB 5对游离氯和臭氧的敏感性显著低于实验室菌株Faulkner所属的基因组A。基因组B中的衣壳蛋白含有较低数量的含硫氨基酸，容易与氧化剂反应。我们重新制定了Chick-Watson模型，将失活速率常数的概率分布，以捕捉异质性。该扩展的Chick-Watson模型表明，与Faulkner菌株的预测相比，需要高达4.2倍的游离氯CT来实现CVB 5的6-log灭活。因此，建议将消毒能力的变化纳入预测某种类型病毒的总体灭活。
The disinfection susceptibilities of viruses vary even among variants, yet the inactivation efficiency of a certain virus genotype, species, or genus was determined based on the susceptibility of its laboratory strain. The objectives were to evaluate the variability in susceptibilities to free chlorine, UV254, and ozone among 13 variants of coxsackievirus B5 (CVB5) and develop the model allowing for predicting the overall inactivation of heterogeneous CVB5. Our results showed that the susceptibilities differed by up to 3.4-fold, 1.3-fold, and 1.8-fold in free chlorine, UV254, and ozone, respectively. CVB5 in genogroup B exhibited significantly lower susceptibility to free chlorine and ozone than genogroup A, where the laboratory strain, Faulkner, belongs. The capsid protein in genogroup B contained a lower number of sulfur-containing amino acids, readily reactive to oxidants. We reformulated the Chick–Watson model by incorporating the probability distributions of inactivation rate constants to capture the heterogeneity. This expanded Chick–Watson model indicated that up to 4.2-fold larger free chlorine CT is required to achieve 6-log inactivation of CVB5 than the prediction by the Faulkner strain. Therefore, it is recommended to incorporate the variation in disinfection susceptibilities for predicting the overall inactivation of a certain type of viruses.