A complex genetic network involving a broad-spectrum locus and strain-specific loci controls resistance to different pathotypes of Aphanomyces euteiches in Medicago truncatula
A complex genetic network involving a broad-spectrum locus and strain-specific loci controls resistance to different pathotypes of Aphanomyces euteiches in Medicago truncatula
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DOI：
10.1007/s00122-009-1224-x
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发表时间：
2010-03-01
影响因子：
5.4
通讯作者：
Pilet-Nayel, Marie-Laure
中科院分区：
文献类型：
--
作者：
Hamon, Celine;Baranger, Alain;Pilet-Nayel, Marie-Laure
更好地了解植物部分抗性的遗传和基因组基础及其病原体变异性的多样性，是可持续种植系统中抗性遗传因素更持久管理的必要条件。在这项研究中，我们研究了截形苜蓿对真丝酵母（Aphanomyceseuteiches）（一种对豌豆和苜蓿非常有害的病原体）的部分抗性所涉及的遗传因素的多样性。利用F83005.5（感病）与DZA 045.5（抗病）杂交的178个重组自交系群体，对4种小麦赤霉病的抗性进行了QTL定位。euteiches参考菌株属于豌豆和苜蓿上目前已知的四种主要致病型。一个主要的广谱基因组区域，以前被命名为AER 1，定位于3号染色体上一个减少的440 kb间隔，并参与完全或部分抗性，这取决于A。Euteiches菌株我们还鉴定了21个加性和/或上位基因组区域，这些区域对一个或两个菌株具有特异性，其中几个被锚定到M。truncatula物理图。这些结果表明，在M. truncatula是一个复杂的遗传位点网络，控制着对不同豌豆和苜蓿病原型的部分抗性。euteiches，表明多样性的分子机制的基础上部分电阻。
A higher understanding of genetic and genomic bases of partial resistance in plants and their diversity regarding pathogen variability is required for a more durable management of resistance genetic factors in sustainable cropping systems. In this study, we investigated the diversity of genetic factors involved in partial resistance to Aphanomyces euteiches, a very damaging pathogen on pea and alfalfa, in Medicago truncatula. A mapping population of 178 recombinant inbred lines, from the cross F83005.5 (susceptible) and DZA045.5 (resistant), was used to identify quantitative trait loci for resistance to four A. euteiches reference strains belonging to the four main pathotypes currently known on pea and alfalfa. A major broad-spectrum genomic region, previously named AER1, was localized to a reduced 440 kb interval on chromosome 3 and was involved in complete or partial resistance, depending on the A. euteiches strain. We also identified 21 additive and/or epistatic genomic regions specific to one or two strains, several of them being anchored to the M. truncatula physical map. These results show that, in M. truncatula, a complex network of genetic loci controls partial resistance to different pea and alfalfa pathotypes of A. euteiches, suggesting a diversity of molecular mechanisms underlying partial resistance.