Effect of several vasoactive agents on guanylate cyclase activity in isolated rat brain microvessels.
Effect of several vasoactive agents on guanylate cyclase activity in isolated rat brain microvessels.
复制标题
几种血管活性剂对离体大鼠脑微血管鸟苷酸环化酶活性的影响。
DOI：
10.1016/0304-3940(89)90830-6
复制
发表时间：
1989
影响因子：
2.5
通讯作者：
Harik,SI
中科院分区：
文献类型：
--
作者：
Homayoun,P;Lust,WD;Harik,SI
我们测试了以下可能的血管活性物质在10−3到10−9M的浓度范围内刺激大鼠脑微血管鸟苷环化酶活性的能力：血管紧张素II、精氨酸加压素、心钠素、缓激动素、卡巴胆碱和凝血酶。在硝普钠存在的情况下，确定了脑微血管增加其循环GMP生成的能力。在所有被测试的药物中，只有心钠素能刺激大鼠脑微血管循环GMP的产生。这种刺激呈剂量依赖性，在1μM浓度时达到最大。这些结果与脑微血管心钠素受体的发现一致，提示心钠素对构成血脑屏障的脑毛细血管的功能具有重要的调节作用。如果其他血管活性物质的受体存在于脑微血管中，它们的作用似乎不是由作为第二信使的环状GMP介导的。
We tested the ability of the following putative vasoactive agents to stimulate guanylate cyclase activity in isolated rat cerebral microvessels: angiotensin II, arginine vasopressin, atrial natriuretic peptide, bradykinin, carbachol and thrombin; at concentrations ranging between 10−3and 10−9M. The ability of cerebral microvessels to increase their cyclic GMP generation was ascertained in the presence of sodium nitroprusside. Of all the agents tested, only atrial natriuretic peptide stimulated cyclic GMP generation in isolated rat cerebral microvessels. Such stimulation was dose-dependent, reaching its maximum at 1 μM concentration. These results are consistent with the finding of atrial natriuretic peptide receptors in brain microvessels, and suggest that this peptide has an important role in modulating the function of brain capillaries, which constitute the blood-brain barrier. If receptors for the other vasoactive agents exist in brain microvessels, their action does not seem to be mediated by cyclic GMP as a second messenger.