PRODUCTS OF THE IRON-MOLYBDENUM COFACTOR-SPECIFIC BIOSYNTHETIC GENES, NIFE AND NIFN, ARE STRUCTURALLY HOMOLOGOUS TO THE PRODUCTS OF THE NITROGENASE MOLYBDENUM-IRON PROTEIN GENES, NIFD AND NIFK
PRODUCTS OF THE IRON-MOLYBDENUM COFACTOR-SPECIFIC BIOSYNTHETIC GENES, NIFE AND NIFN, ARE STRUCTURALLY HOMOLOGOUS TO THE PRODUCTS OF THE NITROGENASE MOLYBDENUM-IRON PROTEIN GENES, NIFD AND NIFK
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DOI：
10.1128/jb.169.4.1547-1553.1987
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发表时间：
1987-04-01
影响因子：
3.2
通讯作者：
DEAN, DR
中科院分区：
文献类型：
--
作者：
BRIGLE, KE;WEISS, MC;DEAN, DR
维氏固氮菌的基因与肺炎克雷伯菌的铁钼辅因子生物合成基因 nifE 和 nifN 同源，现已被克隆和测序。这些基因包含单个转录单位，位于固氮酶结构基因簇 (nifHDK) 的下游。 DNA序列分析表明，NifE和nifN基因的产物分别与nifD(MoFe蛋白α亚基)和nifK(MoFe蛋白β亚基)基因产物相比具有相当大的同源性。这些惊人的序列同源性表明了所提出的 nifEN 产物复合物与固氮酶 MoFe 蛋白之间的结构和功能关系，并暗示了这些基因簇之间的祖先关系。 nifEN 基因簇内含有缺失的菌株的分离和表征证明了这些产物在 A. vinelandii 铁钼辅因子生物合成中的作用。
The genes from Azotobacter vinelandii, which are homologous to the iron-molybdenum cofactor biosynthetic genes, nifE and nifN, from Klebsiella pneumoniae, have been cloned and sequenced. These genes comprise a single transcription unit and are located immediately downstream from the nitrogenase structural gene cluster (nifHDK). DNA sequence analysis has revealed that the product of the NifE and nifN genes contain considerable homology when compared with the nifD (MoFe protein .alpha. subunit) and the nifK (MoFe protein .beta. subunit) gene products, respectively. These striking sequence homologies indicate at structural and functional relationship between a proposed nifEN product complex and the nitrogenase MoFe protein as well as imply an ancestral relationship between these gene clusters. The isolation and characterization of strains which contain deletions within the nifEN gene cluster demonstrate a role for these products in iron-molybdenum cofactor biosynthesis in A. vinelandii.