Differential regulation of brain-derived neurotrophic factor messenger RNA cellular expression in the adult rat visual cortex
Differential regulation of brain-derived neurotrophic factor messenger RNA cellular expression in the adult rat visual cortex
复制标题
DOI：
10.1016/s0306-4522(99)00240-7
复制
发表时间：
1999-01-01
期刊：
影响因子：
3.3
通讯作者：
Domenici, L
中科院分区：
文献类型：
--
作者：
Capsoni, S;Tongiorgi, E;Domenici, L
在这项研究中，我们报告了在单眼剥夺的关键期（出生后第35天）和成年期（出生后第90天）结束时分析的大鼠双眼初级视觉皮层中脑源性神经营养因子信使RNA的分布的比较分析。高分辨率非同位素原位杂交结合尼氏染色，可以确定表达脑源性神经营养因子信使RNA的神经元的相对数量。在出生后第90天的大鼠，脑源性神经营养因子信使RNA阳性的神经元的相对数量显着减少，在第II/III层相对于出生后第35天的动物，是恒定的，在所有其他皮质层。此外，我们证明，黑暗饲养22天，从出生后第90天开始，确定：（i）降低脑源性神经营养因子信使RNA的整体水平，从而降低标记强度的所有细胞在整个皮质层II-VI;（ii）增加细胞数量表达脑源性神经营养因子信使RNA在IV和V层;和（iii）暗饲养后树突中脑源性神经营养因子信使RNA的染色强度降低。黑暗期后再次暴露于光2小时几乎恢复了脑源性神经营养因子RNA阳性神经元的数量。我们得出结论，脑源性神经营养因子信使RNA在第II/III层神经元中的成熟超过出生后35-40天，这可以被认为是关键期的结束[Fagiolini M.等人（1994）维斯.结果：34，709-720]。此外，我们表明，脑源性神经营养因子信使RNA的细胞表达是由光在成年大鼠以及在发展过程中。(C)1999年IBRO。出版社：Elsevier Science Ltd
In this study, we report a comparative analysis of the distribution of brain-derived neurotrophic factor messenger RNA in the binocular primary visual cortex of rats analysed at the end of the critical period for monocular deprivation (postnatal day 35) and during adulthood (postnatal day 90). High-resolution non-isotopic ia situ hybridization coupled with Nissl staining allowed to determine the relative number of neurons expressing brain-derived neurotrophic factor messenger RNA. In postnatal day 90 rats, the relative number of neurons positive for brain-derived neurotrophic factor messenger RNA significantly decreases in layer II/III with respect to postnatal day 35 animals, being constant in all the other cortical layers. Moreover, we demonstrate that dark rearing for 22 days, starting from postnatal day 90, determines: (i) a decrease of the overall level of brain-derived neurotrophic factor messenger RNA with a consequent reduction of labelling intensity in all cells throughout cortical layers II-VI; (ii) an increase of cell numbers expressing brain-derived neurotrophic factor messenger RNA in layers IV and V; and (iii) a decreased intensity of staining for brain-derived neurotrophic factor messenger RNA in dendrites after dark rearing. A re-exposure to light for 2 h after the period of darkness almost restores the number of brain-derived neurotrophic factor RNA-positive neurons. We conclude that the maturation of brain-derived neurotrophic factor messenger RNA in neurons of layer II/III goes beyond postnatal days 35-40, which can be considered the end of the critical period [Fagiolini M. et al. (1994) Vis. Res., 34, 709-720]. Moreover, we show that the cellular expression of brain-derived neurotrophic factor messenger RNA is regulated by light in adult rats as well as during development. (C) 1999 IBRO. Published by Elsevier Science Ltd.