Cloning and characterization of alpha PS1, a novel Drosophila melanogaster integrin.
Cloning and characterization of alpha PS1, a novel Drosophila melanogaster integrin.
复制标题
α PS1（一种新型果蝇整合素）的克隆和表征。
作者：
M. Wehrli;A. Diantonio;I. Fearnley;R. J. Smith;M. Wilcox
果蝇位置特异性整联蛋白（PS整联蛋白或PS抗原）包含两个异二聚体复合物，αPS1βPS和αPS2βPS。通过此处描述的 α PS1 克隆，我们完成了三个 PS 整合素亚基一级结构的表征。我们纯化了αPS1亚基，获得了肽序列并分离了基因组和cDNA克隆。编码的 α PS1 蛋白包含切割的 α 整合素的半胱氨酸模式、三个假定的金属结合域，并显示 α 整合素的其他特征。序列变异区域表明 α PS1 与所有其他 α 链不同。转录本分析显示，α PS1 mRNA 和蛋白质表达模式相同，表明该基因受转录控制。我们比较了果蝇 α PS1、α PS2、人类 α IIb 和 α X (p150,95) 以及秀丽隐杆线虫 F54G8.3 整合素的基因结构。我们发现内含子的几个位置和阶段是保守的，这也得到了氨基酸序列中保守性的支持，表明它们都源自一个共同的祖先基因。
The Drosophila position-specific integrins (PS integrins or PS antigens) comprise two heterodimeric complexes, alpha PS1 beta PS and alpha PS2 beta PS. With the cloning of alpha PS1 described here, we complete the characterization of the primary structure of the three PS integrin subunits. We have purified the alpha PS1 subunit, obtained peptide sequence and isolated genomic and cDNA clones. The encoded alpha PS1 protein contains the cysteine pattern of the cleaved alpha integrins, three putative metal binding domains and shows the other characteristic features of alpha integrins. Regions of sequence variation indicate that alpha PS1 is distinct from all other alpha chains. The transcript analysis shows that the patterns of both alpha PS1 mRNA and protein expression are the same, suggesting that the gene is controlled transcriptionally. We compare the gene structures of the Drosophila alpha PS1, alpha PS2, the human alpha IIb and alpha X (p150,95) and the C. elegans F54G8.3 integrins. We find several positions and phases of introns conserved which, supported by conservation also in the amino acid sequence, indicates that they all derive from a common ancestral gene.