Distinct cell surface ligands mediate T lymphocyte attachment and rolling on P and E selectin under physiological flow.
Distinct cell surface ligands mediate T lymphocyte attachment and rolling on P and E selectin under physiological flow.
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DOI：
10.1083/jcb.127.5.1485
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发表时间：
1994-12
期刊：
影响因子：
--
通讯作者：
Kupper TS
中科院分区：
文献类型：
--
作者：
Alon R;Rossiter H;Wang X;Springer TA;Kupper TS
记忆T淋巴细胞在炎症部位外渗，但这些细胞启动与内皮细胞接触和束缚的机制尚不完全清楚。白细胞外渗的一个重要部分是内皮选择素上滚动粘附的启动;已在单核细胞和中性粒细胞中研究了此类事件，但未在淋巴细胞中研究。在这项研究中，潜在的T淋巴细胞粘附和滚动的内皮选择素在体外进行了研究。我们证明，T细胞可以形成栓系和滚动粘附P选择素和E选择素在生理流动条件下。P选择素上的束缚和滚动不依赖于细胞表面皮肤淋巴细胞抗原（CLA）表达，CLA表达与T细胞在E选择素上流动下形成滚动粘附的能力严格相关。通过溶血性巴斯德菌O-糖蛋白酶选择性去除细胞表面唾液酸粘蛋白，T细胞与P选择素的束缚被消除，而皮肤淋巴细胞抗原表达不受影响。与中性粒细胞和HL-60细胞上的主要P选择素配体P选择素糖蛋白配体-1（PSGL-1）相同或密切相关的唾液粘蛋白分子似乎是P选择素的主要T细胞配体。P选择素糖蛋白配体-1似乎不支持T细胞在E选择素上滚动。反过来，E选择素配体似乎与唾液粘蛋白无关。这些数据证明了T细胞上P或E选择素的结构不同的配体的存在，提供了两种配体可以在单个T细胞上共表达的证据，并以相互排斥的方式介导了各自选择素的束缚和滚动。
Memory T lymphocytes extravasate at sites of inflammation, but the mechanisms employed by these cells to initiate contact and tethering with endothelium are incompletely understood. An important part of leukocyte extravasation is the initiation of rolling adhesions on endothelial selectins; such events have been studied in monocytes and neutrophils but not lymphocytes. In this study, the potential of T lymphocytes to adhere and roll on endothelial selectins in vitro was investigated. We demonstrate that T cells can form tethers and rolling adhesions on P selectin and E selectin under physiologic flow conditions. Tethering and rolling on P selectin was independent of cell- surface cutaneous lymphocyte antigen (CLA) expression, which correlated strictly with the capacity of T cells to form rolling adhesions under flow on E selectin. T cell tethering to P selectin was abolished by selective removal of cell surface sialomucins by a P. haemolytica O- glycoprotease, while cutaneous lymphocyte antigen expression was unaffected. A sialomucin molecule identical or closely related to P selectin glycoprotein ligand-1 (PSGL-1), the major P selectin ligand on neutrophils and HL-60 cells, appears to be a major T cell ligand for P selectin. P selectin glycoprotein ligand-1 does not appear to support T cell rolling on E selectin. In turn, E selectin ligands do not appear to be associated with sialomucins. These data demonstrate the presence of structurally distinct ligands for P or E selectins on T cells, provide evidence that both ligands can be coexpressed on a single T cell, and mediate tethering and rolling on the respective selectins in a mutually exclusive fashion.