Determination of the nucleoside triphosphate contents of eggs and oocytes of Xenopus laevis.
Determination of the nucleoside triphosphate contents of eggs and oocytes of Xenopus laevis.
复制标题
非洲爪蟾卵和卵母细胞中三磷酸核苷含量的测定。
作者：
H. Woodland;R. Pestell
本文测定了非洲爪蟾卵和卵母细胞中核糖核苷酸和脱氧核糖核苷酸的含量。鸡蛋含有大多数脱氧核糖核苷酸的三磷酸盐的形式。dCTP、dTTP、dATP和dGTP以相似的量存在。卵含有足够的脱氧核苷酸三磷酸，以产生约2500个细胞核。卵母细胞中含有较少的嘧啶脱氧核苷三磷酸比鸡蛋，嘌呤脱氧核苷三磷酸未检测到。这些差异可能与卵子诱导核DNA合成的能力有关，这是卵母细胞所没有的。卵母细胞和卵子似乎都含有非磷酸化的α-未取代醛，可能是脱氧核糖衍生物。卵子和卵母细胞含有相似数量的核糖核苷三磷酸。因此，在卵子中发现的RNA合成速率低，而不是在卵母细胞中，不是由简单的前体控制引起的。
The ribonucleotide and deoxyribonucleotide contents of eggs and oocytes of Xenopus laevis were measured. Eggs contained most deoxyribonucleotide in the form of triphosphates. dCTP, dTTP, dATP and dGTP were present in similar amounts. The egg contained sufficient deoxynucleotide triphosphate to make approximately 2500 nuclei. Oocytes contained less pyrimidine deoxyribonucleoside triphosphates than did eggs, and purine deoxyribonucleoside triphosphates were not detected. These differences may be correlated with the ability of eggs to induce nuclear DNA synthesis, a property not shown by oocytes. Both oocytes and eggs seem to contain non-phosphorylated, alpha-unsubstituted aldehydes, which may be deoxyribose derivatives. Eggs and oocytes contain similar amounts of ribonucleoside triphosphates. The low rate of RNA synthesis found in eggs, but not in oocytes, is therefore not caused by simple precursor control.