Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization
Enhanced discrimination of single nucleotide polymorphisms by artificial mismatch hybridization
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DOI：
10.1038/nbt0497-331
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发表时间：
1997-04-01
影响因子：
46.9
通讯作者：
Smith, LM
中科院分区：
文献类型：
--
作者：
Guo, Z;Liu, QH;Smith, LM
为了提高DNA杂交中单核苷酸多态性的区分度，使用碱基类似物3-硝基吡咯将人工错配插入探针寡核苷酸中。与常规杂交相比，用正常和单核苷酸变体DNA靶形成的杂交体之间的热稳定性差异（Δ T-m）增加多达200%，并且强烈依赖于错配之间的间隔。通过杂交分析和HLA-DRB基因座内的等位基因特异性扩增证明了特异性的增加。
In order to increase the discrimination of single nucleotide polymorphisms in DNA hybridization, artificial mismatches are inserted into probe oligonucleotides using the base analog 3-nitropyrrole. Differences in thermal stability (Delta T-m) between hybrids formed with normal and single-nucleotide-variant DNA targets are increased by as much as 200% over conventional hybridization, and are strongly dependent upon the spacing between mismatches. The increased specificity is demonstrated by hybridization analysis and allele-specific amplification within the HLA-DRB locus.