Inhibition of angiogenesis and tumor growth by a synthetic laminin peptide, CDPGYIGSR-NH2.
Inhibition of angiogenesis and tumor growth by a synthetic laminin peptide, CDPGYIGSR-NH2.
复制标题
合成层粘连蛋白肽 CDPGYIGSR-NH2 抑制血管生成和肿瘤生长。
作者：
N. Sakamoto;M. Iwahana;Noriko G. Tanaka;Yasuaki Osada
层粘连蛋白衍生的合成肽Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH 2（CDPGYIGSR-H2）含有用于细胞结合的活性位点，其抑制血管生成和实体瘤生长。它有效地抑制胚胎血管生成的鸡绒毛尿囊膜和迁移的血管内皮细胞诱导的肿瘤条件培养基，但既不是在体外增殖的内皮细胞，也不是肿瘤细胞。此外，在体内试验中，CDPGYIGSR-NH 2显著抑制s.c.肉瘤180实体瘤和刘易斯肺癌（3LL）。相反，肉瘤180的腹水肿瘤生长不受该肽的影响，即使使用相同的细胞来源。由此得出结论，CDPGYIGSR-NH 2对实体瘤生长的抑制不是由于对细胞生长的直接影响，而是由于通过抑制内皮细胞迁移介导的抗血管生成作用。
A laminin-derived synthetic peptide, Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-NH2 (CDPGYIGSR-H2), containing an active site for cell binding inhibited both angiogenesis and solid tumor growth. It potently suppressed both embryonic angiogenesis of the chick chorioallantoic membrane and migration of vascular endothelial cells induced by a tumor-conditioned medium but neither the in vitro proliferation of endothelial cells nor that of tumor cells. Additionally, in in vivo tests, CDPGYIGSR-NH2 markedly inhibited both the growth of s.c. solid tumor of Sarcoma 180 and that of Lewis lung carcinoma (3LL) in the lungs. On the contrary, ascitic tumor growth of Sarcoma 180 was not affected by this peptide, even though the same cell source was used. It was concluded that solid tumor growth inhibition by CDPGYIGSR-NH2 was due not a direct effect on cell growth but to antiangiogenic effect mediated by the inhibition of endothelial cell migration.