Buffered Platelet-Rich Plasma Enhances Mesenchymal Stem Cell Proliferation and Chondrogenic Differentiation
Buffered Platelet-Rich Plasma Enhances Mesenchymal Stem Cell Proliferation and Chondrogenic Differentiation
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DOI：
10.1089/ten.tec.2008.0534
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发表时间：
2009-09-01
影响因子：
3
通讯作者：
Jacobs, Christopher R.
中科院分区：
文献类型：
--
作者：
Mishra, Allan;Tummala, Padmaja;Jacobs, Christopher R.
通过添加可增加祖细胞或干细胞增殖和分化的辅助剂，可以显着提高组织工程应用的成功率。富血小板血浆（PRP）最近已成为治疗急性和慢性肌腱疾病的潜在生物工具。 PRP 的再生潜力基于血小板破裂时生长因子的释放。其自体性质使其在组织工程应用中具有显着优势。为了测试 PRP 是否对软骨再生特别有用，设计了一种细胞培养实验，其中间充质干细胞 (MSC) 在对照培养基或用灭活的缓冲 PRP 增强的培养基中生长。 PRP 处理后 7 天的增殖增加：对照培养基细胞为 1.041，而对照培养基细胞为 0.199（p
The success of tissue engineering applications can potentially be dramatically improved with the addition of adjuncts that increase the proliferation and differentiation of progenitor or stem cells. Platelet-rich plasma (PRP) has recently emerged as a potential biologic tool to treat acute and chronic tendon disorders. The regenerative potential of PRP is based on the release of growth factors that occurs with platelet rupture. Its autologous nature gives it a significant advantage in tissue engineering applications. To test whether PRP may be useful specifically for cartilage regeneration, a cell culture experiment was devised in which mesenchymal stem cells (MSCs) were grown in control media or media enhanced with inactivated, buffered PRP. Proliferation 7 days after PRP treatment was increased: 1.041 versus 0.199 for the control media cells (p