In vivo colonization of salivary pellicle by Haemophilus, Actinomyces and Streptococcus species.
In vivo colonization of salivary pellicle by Haemophilus, Actinomyces and Streptococcus species.
复制标题
嗜血杆菌、放线菌和链球菌在唾液膜的体内定殖。
DOI：
10.1159/000260979
复制
发表时间：
1986
期刊：
影响因子：
4.2
通讯作者：
Bloomquist,CG
中科院分区：
文献类型：
--
作者：
Liljemark,WF;Fenner,LJ;Bloomquist,CG
对8名口腔健康的受试者进行了牙菌斑形成的研究。在对4个上前磨牙和2个第一磨牙进行牙面浮磨和表面清洁之前，采集牙周上菌斑、颊粘膜、舌背和唾液。样品在对口腔链球菌属、嗜血杆菌属和放线菌属有选择性的培养基上进行厌氧处理，并补充血液琼脂以鉴定革兰氏阴性菌群和总可培养菌群。大多数口腔标本中检出嗜血杆菌、血链球菌、中型链球菌、变形链球菌和唾液链球菌。对洗牙前口腔样本中放线菌、粘性放线菌、奈氏放线菌和溶牙放线菌的分布进行分析，发现唾液、颊粘膜、舌头或菌斑样本中这些菌种的比例或流行率没有显著差异。在所有受试者的大多数表面上都发现了这三种物种。在清洁后，菌斑被允许形成48小时。当一种菌斑在2小时菌斑中的比例高于同一人的唾液样本中的比例时，被解释为由于唾液膜对物种的促进(作为清洁牙齿的2小时菌斑)。唾液和2小时斑块的中位数比率为1：3。建议使用牙膜来促进牙齿的生长。Sanguis显示唾液：2小时菌斑的中位数比例为1：6或更高，这表明唾液膜强烈促进了这些物种的黏附。发展中的斑块含有较高比例的链球菌，尤其是S。血统和S。Mitis，以及副流感嗜血杆菌。OFS的比例显著增加。Sanguisin 48小时与24小时，以及2小时菌斑。在大多数正在形成的斑块样本中都发现了溶牙现象，而且它们之间的比例相似。OFA的比例显著增加了两倍。OFS增加4倍。Sanguis，在48小时和2小时的斑块中被看到，表明这些物种的生长。48小时斑块主要由Facklam 1s组成。这一物种被发现的比例明显高于其他物种。唾液。变种人。需要NAD的血友病比例很高，在48小时斑块中占血友病的99%。虽然在ASA的48小时样本中没有检测到奈氏拟青霉。粘性A。在48小时显影的牙菌斑样本中，这三种细菌的比例相似。
Eight human subjects with healthy oral conditions were examined to study developing dental plaque. Supragingival plaque, buccal mucosa, tongue dorsum and saliva were sampled prior to the pumicing and cleaning of facial surfaces of the four upper premolars and two first molars. Samples were processed anaerobically on media that were selective for the oralStreptococcus spp.,Haemophilus spp., andActinomycesspp., as well as supplemented blood agar for identification of gram-negative bacterial groups and total cultivable flora. NAD-requiringHaemophilusspp.,Streptococcus sanguis,Streptococcus mitis,Streptococcus mutansandStreptococcus salivariuswere found in most oral samples. Analysis of the distribution of theActinomycesspp.,Actinomyces viscosus,Actinomyces naeslundiiandActinomyces odontolyticusin oral samples prior to teeth cleaning, found no significant differences between proportions or prevalence of these species in saliva, buccal mucosa, tongue, or pooled plaque samples. All three species were found on most surfaces in all subjects. Following cleaning, plaque was allowed to develop for 48 h. Proportions of a species in 2-hour plaque, when higher than proportions in the same individual’s saliva samples, were interpreted to be due to a species promotion by salivary pellicle (as 2-hour plaque from cleaned teeth). A median ratio of 1:3 of saliva versus 2-hour plaque was seen forA. odontolyticussuggesting some promotion by pellicle.A. viscosusandS. sanguisshowed median ratios of saliva:2-hour plaque of 1:6 or higher, suggesting that salivary pellicle strongly promoted these species’ adherence. Developing plaques contained high proportions of streptococci, especiallyS. sanguisandS. mitis, as well asHaemophilus parainfluenzae. Significant increases were seen in proportions ofS. sanguisin 48-hour versus 24-hour, and 2-hour plaques.A. viscosus,A. naeslundiiandA. odontolyticuswere found in most developing plaque samples and in similar proportions to each other. A significant twofold increase in proportions ofA. odontolyticus, and fourfold increase ofS. sanguis, was seen in 48- versus 2-hour plaques suggesting growth of these species. Forty-eight-hour plaque was composed primarily of Facklam type 1S. sanguisand this species was found in significantly higher proportions thanS. salivariusorS. mutans. NAD-requiring haemophili were found in high proportions and comprised 99% of the haemophili in 48-hour plaque. Although A. naeslundii was not dectected in as many 48-hour samples asA. viscosusorA. odontolyticus, these three species were found in similar proportions in 48-hour developing plaque samples.