Functional Exosome-Mediated Delivery of Triptolide Endowed with Targeting Properties as Chemotherapy Carriers for Ovarian Carcinoma
Functional Exosome-Mediated Delivery of Triptolide Endowed with Targeting Properties as Chemotherapy Carriers for Ovarian Carcinoma
复制标题
功能性外泌体介导的具有靶向特性的雷公藤甲素递送作为卵巢癌化疗载体
DOI：
10.1166/jbn.2021.3041
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发表时间：
2021-03-01
影响因子：
2.9
通讯作者：
Li, He
中科院分区：
文献类型：
--
作者：
He, Di;Xu, Xiaoyi;Li, He
卵巢癌是世界范围内最致命的妇科恶性肿瘤。另外，雷公藤内酯醇是从中草药雷公藤中提取的活性物质。wilfordii Hook F.，具有抗肿瘤、免疫调节和抗炎作用。近年来，茶多酚因其具有广谱、高效的抗肿瘤活性而受到越来越多的关注。然而，由于其严重的副作用，其临床实用性受到限制。在本研究中，我们构建了外泌体包裹的TP靶向药物递送系统，研究了其抗肿瘤作用和IP：111.93 115。86 On：2021年4月21日星期三07：1351体内和体外机制。我们观察到，与游离TP相比，TP-Exos显著增强了抗卵巢癌作用，并降低了对重要器官的毒性。我们进一步证明TP-Exos诱导卵巢癌细胞凋亡，通过激活线粒体凋亡途径调节肿瘤免疫，并选择性地抑制肿瘤微环境中的M2肿瘤相关巨噬细胞及其促肿瘤介质。总之，TP-Exos是一种有前途的卵巢癌治疗方法。
Ovarian cancer has been the most lethal gynaecological malignancy worldwide. Additionally, triptolide is an active sub-stance that has been extracted from the Chinese herbal medicine T. wilfordii Hook F., which possesses anti-tumor, immunomodulatory and anti-inflammatory properties. In recent years, TP has attracted increasing attention because of its broad-spectrum efficient anti-tumor activity. Nevertheless, its clinical utility is limited due to its severe side effects. In this study, we constructed an exosome-encapsulated TP targeted drug delivery systems, studying its anti-tumor effects and IP: 111.93 115. 86 On: Wed, 21 Apr 2021 07:1351 mechanisms in vivo and in vitro. We observed that compared with free TP, TP-Exos significantly enhanced anti-ovarian Copyright: American Scientific Publishers D li er d by I g t cancer effects and reduce toxicity to important organs. We further demonstrated that TP-Exos induced apoptosis of ovar-ian cancer cells, regulated tumor immunity by activating the mitochondrial apoptosis pathway and selectively inhibited M2 tumor-associated macrophages and their tumor-promoting mediators in the tumor microenvironment. In summary, TP-Exos are a promising treatment for ovarian cancer.